Resistance of Biomphalaria occidentalis from Varzea das Flores dam, Minas Gerais, to Schistosoma mansoni infection detected by low stringency polymerase chain reaction

Memórias do Instituto Oswaldo Cruz
C P Souza, L K Passos

Abstract

Biomphalaria occidentalis Paraense, 1981 from Varzea das Flores dam, MG, Brazil, was exposed to infection with Schistosoma mansoni. Individual infection was performed with 140 B. occidentalis and 100 B. glabrata snails using LE and SJ strains. Two groups of B. occidentalis were killed after seven day-miracidia exposure to detect S. mansoni DNA, through the low stringency polymerase chain reaction (LS-PCR), and were negative. The infection rates were 69.2% (LE strain) and 96.7% (SJ strain) for B. glabrata and 0% for B. occidentalis. LS-PCR enabled early resistance diagnosis.

References

Jan 1, 1982·Memórias do Instituto Oswaldo Cruz·W L Paraense, L R Corrêa
Apr 1, 1981·Memórias do Instituto Oswaldo Cruz·W L Paraense
Apr 1, 1993·Memórias do Instituto Oswaldo Cruz·L C LimaC T Guimarães
Feb 11, 1993·Nucleic Acids Research·E Dias NetoA J Simpson

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Citations

May 11, 2011·Revista Da Sociedade Brasileira De Medicina Tropical·Sandra Helena Cerrato TibiriçáElaine Soares Coimbra
Nov 12, 2002·Memórias do Instituto Oswaldo Cruz·R G M SpadaJ T Ribeiro-Paes

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