PMID: 30146861Aug 1, 2016Paper

Resistance of rAAV2-MfE77.43-Transferred Mice to Schistosoma japonicum Infection

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
Hong-mei WangZhi-qiang Qin

Abstract

To investigate the resistance of E77.43 gene of Microtus fortis(MfE77.43) to Schistosoma japonicum infection. MfE77.43 was constructed into the recombinant Adeno-associated virus AAV2. The AAV2-MfE77.43 was transfected into HEK293 cells by the calcium phosphate DNA coprecipitation method. The recombinant rAAV2-MfE77.43 was purified and total RNA was extracted from the transfected cells. The expression of E77.43 was examined by RT-PCR and the purity of rAAV2-MfE77.43 was analyzed by SDS-PAGE. Eighteen KM mice were divided into three groups (n=6 in each group). Mice in the experiment group were intramuscularly injected on days 0, 3 and 7 with 400 μl recombinant AAV2-MfE77.43 virus which was 5-fold diluted in normal saline. Mice in negative control and blank control groups received same volume of pAAV or normal saline. Venous blood was collected through the tail before each injection, and E77.43 expression in plasma was detected by dot-ELISA method. After the last injection, each mouse was infected with 40 S. japonicum cercariae and sacrificed on day 41 after infection. Adult worms and liver eggs per gram(LEPG) were counted. Worm and egg reduction rate was calculated respectively. Egg granulomas were observed by HE staining. RT-PC...Continue Reading

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