Resistance status of Helicoverpa armigera against Bt cotton in Pakistan

Transgenic Research
Shakeel AhmadJam Nazeer Ahmad

Abstract

Transgenic cotton expressing the toxin Cry1Ac from Bacillus thuringiensis L. (Bt) is widely cultivated in Pakistan after its formal approval in 2010. The exposure of the local target pests to the Cry1Ac endotoxin for this duration might have changed the baseline susceptibility. To probe the status of resistance in one of the main target pests, Helicoverpa armigera, field-collected larvae were reared in the lab for conducting leaf fed bioassays. Twenty-six cotton accessions collected from farmers, including 25 Bt-cotton and one non-Bt, were tested to quantify the level of Cry1Ac, an insecticidal crystalline protein (ICP), in leaves of lower, middle and upper canopies of plants. The concentration of ICP was tested through Enzyme-linked Immunosorbent Assay and found significantly variable (P < 0.01) between and within accessions. The highest mean expression was observed in Accession-2 and Accession-4, while the lowest in Accession-21 and Accession-19. Among fresh leaf tissues from different parts of the plant, the highest mean expression was recorded at 60 days after sowing in upper canopy leaves of cotton accessions, which decreased in lower parts of the plant with the lowest mean expression in lower canopy leaves. Laboratory bio...Continue Reading

References

Jan 5, 2006·Applied and Environmental Microbiology·María A IbargutxiPrimitivo Caballero
Jan 30, 2010·Science·H Charles J GodfrayCamilla Toulmin
Feb 9, 2013·Nature Biotechnology·Guanming ShiJoseph Lauer
Jun 12, 2013·Nature Biotechnology·Bruce E TabashnikYves Carrière
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Mar 5, 2016·Microbiology and Molecular Biology Reviews : MMBR·Maissa ChakrounJuan Ferré
Oct 12, 2017·Nature Biotechnology·Bruce E Tabashnik, Yves Carrière

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Citations

Jan 10, 2020·Briefings in Functional Genomics·Shakeel AhmadShaoqing Tang

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Methods Mentioned

BETA
transgenic
ELISA

Software Mentioned

GenStat
Statistix

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