Abstract
We describe the properties of a physiological cell death (PCD)-resistant subline of WEHI-231 generated from the PCD-susceptible WEHI-231.7 JM cell line maintained in our laboratory. The PCD-resistant WEHI-231.7 JMRE subline was uniquely resistant to anti-immunoglobulin (Ig)M-induced PCD but not to irradiation and etoposide. In these sublines, we compared the expression of genes implicated in regulating PCD. Northern analysis of c-myc, c-fos, egr-1, Fas, p53 and retinoblastoma revealed similar basal levels of expression in all sublines tested and comparable responses to anti-IgM treatment. Similarly, the expression of bcl-2, bcl-x, bax and IL-1 beta converting enzyme did not correlate with susceptibility to anti-IgM-induced PCD. Next, we systematically studied signal transduction events including: tyrosine phosphorylation, Ca++ flux, and ceramide production in the Jm and JMRE sublines. The tyrosine phosphorylation patterns and the Ca++ influx generated following sIgM engagement were very similar in the JM and JMRE sublines. In contrast, the generation of ceramide differed in the PCD-resistant and PCD-susceptible sublines. Ceramide is produced following cross-linking sIgM on WEHI-231.7 JM cells and causes PCD. Ceramide levels in ...Continue Reading
References
Mar 26, 1992·Nature·R Watanabe-FukunagaS Nagata
May 1, 1992·Molecular Immunology·N IwabuchiN Hozumi
Jul 26, 1991·Cell·N ItohS Nagata
Mar 1, 1989·The Journal of Experimental Medicine·J G MonroeN Sykes
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M L Cleary, J Sklar
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R TischN Hozumi
Mar 13, 1987·Science·W H LeeE Y Lee
Jul 1, 1985·Molecular and Cellular Biology·E HarlowT E Adams
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J E McCormackG E Sonenshein
Jun 23, 1983·Nature·R WattG Rovera
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F van StraatenI M Verma
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·A R GottschalkJ Quintáns
Jan 1, 1993·The Journal of Experimental Medicine·E RouvierP Golstein
Jul 29, 1994·Biochemical and Biophysical Research Communications·J QuintansG Dawson
Jan 1, 1994·International Immunology·A R GottschalkJ Quintáns
Nov 1, 1993·European Journal of Immunology·U HibnerP Sarthou
Nov 19, 1993·Cell·J YuanH R Horvitz
Nov 19, 1993·Cell·M MiuraJ Yuan
Aug 1, 1993·European Journal of Immunology·A R GottschalkJ Quintáns
Aug 27, 1993·Cell·L H BoiseC B Thompson
Aug 27, 1993·Cell·Z N OltvaiS J Korsmeyer
Sep 24, 1993·Cell·S W LoweD E Housman
Jan 22, 1993·Science·S MathiasR N Kolesnick
Mar 19, 1993·Science·L M ObeidY A Hannun
Apr 29, 1993·Nature·S W LoweT Jacks
Apr 29, 1993·Nature·A R ClarkeA H Wyllie
Citations
Mar 21, 2000·Journal of Neuroscience Research·B Scurlock, G Dawson
Sep 1, 1999·Journal of Neuroscience Research·R GoswamiG Dawson
Mar 31, 2000·Journal of Neuroscience Research·R Goswami, G Dawson
Mar 10, 1998·Journal of Cellular Physiology·A Jelaska, J H Korn
Jun 1, 1996·Current Opinion in Immunology·T L Rothstein
Aug 1, 1997·Immunology·D E MacfarlaneA M Krieg
Aug 1, 1996·The Journal of Experimental Medicine·K MizunoH Yakura
Jan 1, 1997·AIDS·A RichardL Poulin
Oct 15, 1998·Transplantation·E S Woodle, S Kulkarni
Feb 15, 1996·European Journal of Biochemistry·A J HaleG T Williams
Sep 15, 2004·Brain Research·Ryo OhtaniToshiro Okazaki
Aug 10, 2000·FEBS Letters·G DawsonG L Schieven
Sep 1, 1995·European Journal of Immunology·M Y MaparaB Dörken
Jul 1, 1996·European Journal of Immunology·M KashiwadaT Takemori
Sep 13, 2003·Blood·Elad KatzMargaret M Harnett
Jun 11, 1999·Immunology and Cell Biology·D Watters
Mar 28, 1998·The Journal of Biological Chemistry·L GenestierN Bonnefoy-Bérard
Feb 13, 2003·The Journal of Biological Chemistry·Bart-Jan KroesenLou F M H de Leij
Feb 7, 1997·The Journal of Biological Chemistry·M B PaumenT Honjo
Apr 11, 1997·The Journal of Biological Chemistry·D A WiesnerG Dawson
Sep 26, 1997·The Journal of Biological Chemistry·A D TepperW J van Blitterswijk
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marco J HeroldIngolf Berberich
Jun 13, 1998·Progress in Lipid Research·L RiboniG Tettamanti
Dec 14, 2004·Neuroscience·H SatoiT Okazaki