Resistance to Chilo infuscatellus (Lepidoptera: Pyraloidea) in transgenic lines of sugarcane expressing Bacillus thuringiensis derived Vip3A protein.

Molecular Biology Reports
Saman RiazBushra Tabassum

Abstract

Sustainable agriculture requires management of insect pests through resistance development. The biological potential of Cry toxins and Vip protein, derived from Bacillus species, is widely recognized in this context. The identification, evaluation of new insecticidal protein genes with different mode of action and entomotoxicity against sugarcane stem borer (Chilo infuscatellus) is important to overcome evolved insect resistance. In this study, we reported the generation of transgenic sugarcane lines expressing Vip3A toxin driven by polyubiquitin promoter for resistance against sugarcane stem borer. The V0 transgenic sugarcane plants were initially characterized by GUS histochemical staining, PCR and Southern blot assays that confirmed genetic transformation of twelve independent sugarcane lines. Variable transgene expression was found among transgenic sugarcane lines when revealed through Realtime quantitative PCR (RT-qPCR) with highest in S10 line while minimum was observed in V5 line. A similar expression pattern was observed in transgenic sugarcane lines for Vip3A protein concentration which ranged from 5.35 to 8.89 µg/mL. A direct correlation was observed between the Vip3A protein and Vip3A transgene expression in the tran...Continue Reading

References

May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·J J EstruchM G Koziel
Mar 6, 1999·Current Opinion in Plant Biology·A J Matzke, M A Matzke
Nov 11, 2008·Journal of Visualized Experiments : JoVE·Alexandrine Froger, James E Hall
Jul 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Bruce E TabashnikYves Carrière
Feb 13, 2013·The Journal of Investigative Dermatology·Lilit Garibyan, Nidhi Avashia
Jun 12, 2013·Nature Biotechnology·Bruce E TabashnikYves Carrière
Feb 11, 2014·Journal of Invertebrate Pathology·Iñigo Ruiz de EscuderoJuan Ferré
Oct 13, 2015·Scientific Reports·Bruce E TabashnikXianchun Li
Dec 5, 2015·Frontiers in Bioengineering and Biotechnology·Nam V HoangRobert J Henry
Jan 18, 2016·Trends in Biotechnology·Yves CarrièreBruce E Tabashnik
Aug 12, 2016·Environmental Science and Pollution Research International·Hong-Xing ChenWang-Peng Shi
Jan 13, 2017·Plant Biotechnology Journal·Thuanne Pires RibeiroMaria Fatima Grossi-de-Sa
Nov 3, 2017·Pest Management Science·Damián A GrimiGraham Head
Apr 11, 2018·Frontiers in Bioengineering and Biotechnology·Adriana Cheavegatti-GianottoWladecir Salles de Oliveira
Jul 8, 2018·Nature Communications·Olivier GarsmeurAngélique D'Hont
Mar 1, 2019·Turkish Journal of Biology = Türk Biyoloji Dergisi·Muhammad TariqTayyab Husnain

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Citations

Apr 25, 2021·Molecular Biotechnology·Aneela IqbalTalaat Ahmed

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Datasets Mentioned

BETA
HM536938.2

Methods Mentioned

BETA
transgenic
PCR
ELISA
enzyme-linked immunosorbent assay
dot blot
genetic

Software Mentioned

[UNK]

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