Resistance to downy mildew in pearl millet is associated with increased phenylalanine ammonia lyase activity

Functional Plant Biology : FPB
N P GeethaH S Shetty

Abstract

Phenylalanine ammonia lyase (PAL) activity was studied in pearl millet cultivars with different levels of resistance to the downy mildew disease caused by Sclerospora graminicola, an important oomycete pathogen. PAL activity was elevated in resistant host cultivar and decreased in susceptible cultivars following downy mildew pathogen infection. The enzyme activation varied between cultivars and was correlated with the degree of resistance to downy mildew disease. The induction of PAL as a response to pathogen inoculation was further corroborated by a time-course study in seedlings and cultured cells of pearl millet. The level of PAL activity was highest at 1.5 h in cultured cells and 4 h in seedlings of resistant host cultivar after inoculation with Sclerospora graminicola. Further studies on PAL activity in different tissues of seedlings showed highest enzyme activity in the young growing region of the root of the resistant host cultivars. The accumulation of wall-bound phenolics and lignin was higher in the resistant cultivar seedlings as evidenced by phloroglucinol-HCl staining and p-coumaric acid assay. The temporal changes in lignin concentration and the concentration of soluble phenolics were greater in root tissues of re...Continue Reading

References

Apr 1, 1982·European Journal of Biochemistry·S E ShieldsC J Lamb
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·E A MaherC J Lamb
Feb 1, 1996·Plant Molecular Biology·T Fukasawa-AkadaJ C Watson
May 18, 2004·The Plant Journal : for Cell and Molecular Biology·Matthew R HemmClint Chapple
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K EdwardsC J Lamb

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Citations

Oct 31, 2012·International Journal of Molecular Sciences·Min GaoXiping Wang
Oct 30, 2013·Molecular Biology Reports·Pushpalatha Hosur GnanaprakashShekar Hunthrike Shetty

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Methods Mentioned

BETA
PAL

Software Mentioned

ICRISAT
JMP

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