PMID: 8460155Mar 15, 1993Paper

Resistance to human immunodeficiency virus 1 infection of SCID mice reconstituted with peripheral blood leukocytes from donors vaccinated with vaccinia gp160 and recombinant gp160

Proceedings of the National Academy of Sciences of the United States of America
D E MosierP D Greenberg

Abstract

SCID mice reconstituted with adult human peripheral blood leukocytes (hu-PBL-SCID mice) make antigen-specific human antibody responses following secondary immunization and can be infected with human immunodeficiency virus 1 (HIV-1), suggesting that they might prove useful for evaluating protective immunity to HIV-1 following vaccination of PBL donors. HIV-seronegative volunteers were immunized with vaccinia expressing HIV-1LAV-1/Bru 160-kDa envelope glycoprotein (vaccinia gp160) and subsequently given booster injections of recombinant gp160 protein (rgp160). Their PBLs were used at intervals of 4-72 weeks after booster injections to construct hu-PBL-SCID mice, which were then challenged with 10(2)-10(3) minimal animal infectious doses of highly homologous HIV-1IIIB. Control hu-PBL-SCID mice were constructed from donors receiving vaccinia, alum, or hepatitis B vaccine. Protection against virus infection was defined as the absence of HIV-1 by culture and no detection of proviral genomes following PCR amplification. Control animals were highly susceptible to HIV infection. By contrast, hu-PBL-SCID mice reconstituted with cells from three of four donors immunized with vaccinia gp160 and recently injected with rgp160 showed no evide...Continue Reading

References

Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M GirardE Muchmore
Feb 15, 1991·Science·D E MosierS A Spector
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C J GibbsJ Salk
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R C DesrosiersM D Daniel
Jan 24, 1985·Nature·L RatnerK Baumeister
Mar 16, 1989·Nature·D E MosierD B Wilson
Dec 23, 1988·Science·R NamikawaJ M McCune
Jul 22, 1988·Science·W C Koff, D F Hoth

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Citations

Sep 1, 1996·Reviews in Medical Virology·M I Johnston
Nov 1, 1995·Immunology Today·M Tary-LehmannP V Lehmann
Feb 1, 1995·International Journal of Immunopharmacology·M Girard
Jan 1, 1993·Biochimie·M Girard
Apr 1, 1994·Current Opinion in Immunology·H KaneshimaJ M McCune
Oct 1, 1994·Current Opinion in Biotechnology·D Ulaeto, D E Hruby
Apr 15, 1999·Immunology Letters·M ClericiS Mazzoli
Jan 1, 1997·Trends in Microbiology·M ClericiG M Shearer
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·M J McElrathJ I Mullins
Oct 31, 2003·AIDS Research and Human Retroviruses·Seth H PincusTamera K Marcotte
Jun 15, 2005·ILAR Journal·Michael K Schunk, G Eileen Macallum
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J LangleyH S Ginsberg
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·D BonnetS R Riddell
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·C ZhangL J Chang
Oct 29, 2014·Expert Opinion on Drug Discovery·Katja Sliva
Aug 4, 1999·Antiviral Chemistry & Chemotherapy·R F SchinaziS Dagan
Nov 17, 2012·Nature Reviews. Microbiology·Theodora Hatziioannou, David T Evans
Sep 15, 1996·Hospital Practice·D E Mosier

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