Resistance to pentamidine is mediated by AdeAB, regulated by AdeRS, and influenced by growth conditions in Acinetobacter baumannii ATCC 17978

PloS One
Felise G AdamsMelissa H Brown

Abstract

In recent years, effective treatment of infections caused by Acinetobacter baumannii has become challenging due to the ability of the bacterium to acquire or up-regulate antimicrobial resistance determinants. Two component signal transduction systems are known to regulate expression of virulence factors including multidrug efflux pumps. Here, we investigated the role of the AdeRS two component signal transduction system in regulating the AdeAB efflux system, determined whether AdeA and/or AdeB can individually confer antimicrobial resistance, and explored the interplay between pentamidine resistance and growth conditions in A. baumannii ATCC 17978. Results identified that deletion of adeRS affected resistance towards chlorhexidine and 4',6-diamidino-2-phenylindole dihydrochloride, two previously defined AdeABC substrates, and also identified an 8-fold decrease in resistance to pentamidine. Examination of ΔadeA, ΔadeB and ΔadeAB cells augmented results seen for ΔadeRS and identified a set of dicationic AdeAB substrates. RNA-sequencing of ΔadeRS revealed transcription of 290 genes were ≥2-fold altered compared to the wildtype. Pentamidine shock significantly increased adeA expression in the wildtype, but decreased it in ΔadeRS, i...Continue Reading

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Citations

May 9, 2019·Future Microbiology·Aimee Rp Tierney, Philip N Rather
Mar 31, 2021·Scientific Reports·Emily M CrossJade K Forwood
Jun 9, 2021·ACS Infectious Diseases·Felise G AdamsBart A Eijkelkamp
Apr 28, 2021·Antimicrobial Agents and Chemotherapy·Vanessa Kornelsen, Ayush Kumar
Oct 29, 2019·ACS Infectious Diseases·Saleh F AlquethamyBart A Eijkelkamp

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Datasets Mentioned

BETA
CP012004
GSE102711

Methods Mentioned

BETA
PCR
RNA-seq
PM2A

Software Mentioned

Hiseq
Biolog
OmniLog Parametric Analysis
OmniLog File Management / Kinetic Analysis

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