Resistance to TGF-beta-induced apoptosis in regenerating hepatocytes

Journal of Cellular Physiology
Blanca HerreraMargarita Fernández

Abstract

Treatment with transforming growth factor beta (TGF-beta) of hepatocytes from two different proliferative conditions, such as fetal development and adult liver regeneration, shows that regenerating cells respond to this cytokine in terms of growth inhibition, but are less sensitive than the fetal ones to the apoptosis induced by this factor. Regenerating TGF-beta treated cells show higher cell viability and lower percentage of apoptotic cells than the fetal treated ones. Furthermore, TGF-beta treated regenerating hepatocytes maintain a well-preserved parenchyma-like organization. Treatment with TGF-beta induces the loss of mitochondrial transmembrane potential in fetal but not in regenerating hepatocytes and activation of caspase-3 is lower in regenerating than in fetal cells. Regenerating hepatocytes show higher intracellular levels of some antiapoptotic proteins, such as Bcl-x(L) and c-IAP-1 and, interestingly, they present higher intracellular glutathione levels, which might provide of mechanisms to avoid potential dangerous effects of the oxidative stress-mediated apoptosis induced by TGF-beta. In fact, treatment with BSO (a glutathione synthesis inhibitor) restores the response of regenerating hepatocytes to TGF-beta in te...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·H L LeffertM Williams
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L BraunN Fausto
Jan 1, 1985·Annual Review of Pharmacology and Toxicology·N KaplowitzM Ookhtens
Nov 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C J Steer
Dec 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·N FaustoE M Webber
Dec 22, 1995·The Journal of Biological Chemistry·V J Thannickal, B L Fanburg
Apr 4, 1997·Science·G K Michalopoulos, M C DeFrances
Jan 13, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Z Z HuangS C Lu
Mar 3, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E R García-TrevijanoM Rojkind
Mar 22, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B HerreraI Fabregat
Oct 27, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D M BissellJ George
Jan 26, 2002·Cell and Tissue Research·Norbert Schuster, Kerstin Krieglstein
Jun 26, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christopher C FranklinNelson Fausto

❮ Previous
Next ❯

Citations

May 14, 2009·World Journal of Gastroenterology : WJG·Magdalena-M DabrowskaRobert Flisiak
Feb 22, 2005·Biochemical and Biophysical Research Communications·J Brian ClarkDavid A Gerber
Apr 20, 2012·World Journal of Experimental Medicine·Mohamed HassanOla Abdelkader
Mar 2, 2017·Cold Spring Harbor Perspectives in Biology·Joan Seoane, Roger R Gomis
Dec 7, 2005·Journal of Cellular Physiology·Irene Carmona-CuencaIsabel Fabregat
Feb 15, 2019·Irish Journal of Medical Science·Mohammad Bagher MahmoudiMahdi Mahmoudi
Jan 7, 2015·The Journal of Biological Chemistry·Genaro Vázquez-VictorioMarina Macías-Silva
Jan 26, 2016·The FEBS Journal·Isabel FabregatUNKNOWN IT-LIVER Consortium
Jan 26, 2021·Redox Biology·M Herranz-ItúrbideI Fabregat
May 4, 2021·Gastroenterology·Nancy R GoughLopa Mishra
Oct 28, 2021·International Journal of Radiation Biology·Yiting TangYang Jiao

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2021 Meta ULC. All rights reserved