Resistance towards metronidazole in Blastocystis sp.: A pathogenic consequence

PloS One
Arutchelvan RajamanikamSuresh Kumar

Abstract

Blastocsytis sp. is a protozoan parasite that has been linked to common gastrointestinal illnesses. Metronidazole, the first line therapy, was reported to show frequent inefficacy. Previously, Blastocystis sp. isolated from different population showed varying metronidazole resistance. However, the effect of metronidazole treatment on pathogenic potentials of Blastocystis sp. isolated from different populations, which is known to have different gut environment, is unclear. This study investigates the in vitro effect of metronidazole on the pathogenic potentials of Blastocystis sp. isolated from urban and orang asli individuals. Blastocystis sp. ST 3 isolated from symptomatic and asymptomatic individuals were treated with a range of metronidazole concentration. The parasites' growth characteristics, apoptotic rate, specific protease activity and the ability to proliferate cancer cells were analyzed upon treatment with 0.001 mg/l metronidazole. The study demonstrates that Blastocystis sp. isolates showed increase in the parasite numbers especially the amoebic forms (only in urban isolates) after treating with metronidazole at the concentration of 0.001 mg/ml. High number of cells in post-treated isolates coincided with increase of...Continue Reading

References

Jan 1, 1993·Annual Review of Microbiology·J H McKerrowJ Bouvier
Jun 5, 1999·Tropical Medicine & International Health : TM & IH·K HareshS Saminathan
Aug 2, 2000·Journal of Clinical Microbiology·N A KhanT A Paget
Mar 11, 2003·Annals of Tropical Medicine and Parasitology·S LeelayoovaM Mungthin
Mar 26, 2003·Journal of Travel Medicine·Luciano NigroBruno Cacopardo
Oct 16, 2004·Digestive Diseases and Sciences·M CandelliAntonio Gasbarrini
Sep 10, 2005·Parasitology Research·Manoj K PuthiaKevin S W Tan
Mar 7, 2006·Parasitology Research·Selena W S SioKevin S W Tan
Jul 4, 2006·Parasitology Research·T C Tan, K G Suresh
Jul 26, 2006·Journal of Pediatric Surgery·Nesibe AndiranFatih Andiran
Jan 8, 2008·Acta Dermato-venereologica·Alexandra Katsarou-KatsariNicholas Vakalis
Mar 20, 2008·Annals of Tropical Medicine and Parasitology·C R StensvoldS Thorsen
Jul 12, 2008·FEMS Microbiology Letters·Ricky DudleyNaveed Ahmed Khan
Oct 17, 2009·Journal of Clinical Gastroenterology·Christen R StensvoldRebecca J Traub
Jan 23, 2010·American Journal of Clinical Pathology·Constantine M VassalosNicholas Vakalis
Feb 19, 2010·Parasitology Research·Samudi ChandramathiUmah Rani Kuppusamy
Apr 7, 2010·Parasitology International·Christian VogelbergChristoph Pöhlmann
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Carlotta De FilippoPaolo Lionetti
Mar 29, 2011·Genome Biology·France DenoeudHicham El Alaoui
Jun 3, 2011·The American Journal of Tropical Medicine and Hygiene·Christen Rune StensvoldHenrik Vedel Nielsen
Nov 15, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Christina M CoyleHerbert B Tanowitz
Mar 10, 2012·Parasitology International·Ivan WawrzyniakHicham El Alaoui
Jun 29, 2012·Trends in Parasitology·Pauline D Scanlan
Jun 29, 2012·Case Reports in Gastroenterology·Mitanshu ShahPaul Mustacchia
Nov 13, 2012·Transactions of the Royal Society of Tropical Medicine and Hygiene·D B DhurgaS Chandramathi
Nov 22, 2012·Gut and Liver·Alexander R MoschenHerbert Tilg
Aug 13, 2013·Parasitology Research·Vinoth KumarasamySuresh Kumar
Aug 21, 2013·Tropical Parasitology·Uma Sekar, M Shanthi
Nov 19, 2013·Journal of Medical Microbiology·Tamalee RobertsDamien Stark
Feb 7, 2014·Parasites & Vectors·Arutchelvan Rajamanikam, Suresh Kumar Govind
Apr 3, 2014·Gut Microbes·Alexander V TyakhtVadim M Govorun
Jun 3, 2014·Gut Pathogens·Tamalee RobertsJohn Ellis
Sep 2, 2014·Parasites & Vectors·Nanthiney Devi RagavanSanjiv Mahadeva
Jun 18, 2015·Parasites & Vectors·Sonal GirishNorhaniza Aminudin

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Citations

May 7, 2019·International Journal of Environmental Research and Public Health·Amira B MokhtarPanagiotis Karanis
Aug 30, 2020·Environment International·Monika MortimerPatricia A Holden
May 4, 2021·Saudi Journal of Biological Sciences·Shereen F MossallamSafaa I Khedr
Jun 8, 2021·Revista de gastroenterología de México·R Contreras-OmañaJ A Velarde-Ruiz Velasco
Jun 24, 2021·Revista De Gastroenterología De México·R Contreras-OmañaJ A Velarde-Ruiz Velasco

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Methods Mentioned

BETA
Assay
Light microscopy
Fluorescence

Software Mentioned

SPSS Statistics

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