PMID: 2488636Jan 1, 1989Paper

Resolution and binding site determination of D,L-thyronine by high-performance liquid chromatography using immobilized albumin as chiral stationary phase. Determination of the optical purity of thyroxine in tablets

Journal of Pharmaceutical and Biomedical Analysis
L DalgaardJ L Pedersen

Abstract

Human and bovine serum albumin bound to silica or aminopropyl silica were used as chiral stationary phases (CSPs). D,L-Thyronine, D,L-tryptophan, N-benzoyl-D,L-phenylalanine, D,L-warfarin and D,L-benzoin could be resolved on these CSPs using a mobile phase of 0.05 M phosphate buffer, pH 7.0. The capacity factor of D-thyronine was higher than that of L-thyronine. The resolution of D,L-thyronine was completely lost by the presence of bilirubin in the mobile phase, but only little affected by caprylate. By contrast, the resolution of D,L-tryptophan was not affected by bilirubin, but lost by the presence of caprylate. These results are consistent with binding of D-thyronine to the bilirubin binding site and L-tryptophan to the caprylate binding site in albumin, respectively, and suggests that such "displacement chromatography" can be used for the determination of binding sites. The optical purity of D-thyroxine in tablets was determined indirectly after de-iodination by catalytic hydrogenation.

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Citations

Nov 1, 1994·Biomedical Chromatography : BMC·H Y Aboul-Enein, V Serignese
Jan 1, 1990·Journal of Pharmaceutical and Biomedical Analysis·A BerthodD W Armstrong
Dec 31, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Helen GikaWolfgang Lindner
Apr 10, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·David S Hage
Dec 16, 2000·Pharmaceutical Science & Technology Today·T Geetha, S Singh
Oct 29, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·C AndréY C Guillaume
Jul 1, 2011·Analytical Methods : Advancing Methods and Applications·David S HageXiwei Zheng
Apr 1, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·D S Hage, J Austin
May 16, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Claire André, Yves Claude Guillaume
Feb 7, 2012·Journal of Pharmaceutical and Biomedical Analysis·David S HageXiwei Zheng

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