Resolution by DEAE-cellulose chromatography of the enzymatic steps in the transformation of arachidonic acid into 8, 11, 12- and 10, 11, 12-trihydroxy-eicosatrienoic acid by the rat lung

Prostaglandins
C R Pace-AsciakS Yamamoto

Abstract

The 30-50% ammonium sulfate fraction of the high speed supernatant (100,000 xg) of a rat lung homogenate is capable of catalysing the conversion of arachidonic acid into 8,11,12- and 10,11, 12-trihydroxyeicosatrienoic acids. This enzyme preparation was resolved through DEAE cellulose chromatography into three stages which were assayed with precursors specific for each stage. Thus in the first stage arachidonic acid is converted by 12-lipoxygenase into 12-hydroperoxy-5,8,10,14-eicosatetraenoic acid (12-HPETE) detected as the corresponding 12-hydroxy product (12-HETE). 12-HPETE in turn is converted into 8-hydroxy-11,12-epoxy-5,9,14-eicosatrienoic acid and 10-hydroxy-11,12-epoxy-5,8,14-eicosatrienoic acid. These epoxides are in turn selectively converted through an epoxide hydrase into the respective triols. While the first and third stages were carried out by distinct fractions from the DEAE columns, the second i.e. conversion of 12-HPETE into epoxides, was detected in all fractions as was the reduction of 12-HPETE into 12-HETE.

References

Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M Hamberg, B Samuelsson
Aug 24, 1981·Biochimica Et Biophysica Acta·C R Pace-AsciakS Yamamoto

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Citations

Oct 19, 2011·Cancer Metastasis Reviews·Cecil R Pace-Asciak
Jul 1, 1993·General Pharmacology·C R Pace-Asciak
Oct 1, 1995·Journal of Lipid Mediators and Cell Signalling·C R Pace-AsciakP Demin
Jan 1, 1996·Journal of Lipid Mediators and Cell Signalling·P M DeminC R Pace-Asciak
Aug 23, 2001·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Y KawanoI Miyakawa
Jul 5, 2007·The FEBS Journal·Santosh NigamRenate Roux-Van der Merwe
Oct 4, 2005·Biochemical and Biophysical Research Communications·Santosh Nigam, Maria-Patapia Zafiriou
Sep 23, 2014·Biochimica Et Biophysica Acta·Cecil R Pace-Asciak

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