PMID: 11607176Apr 15, 1991Paper

Resolution of component proteins in an enzyme complex from Methanosarcina thermophila catalyzing the synthesis or cleavage of acetyl-CoA

Proceedings of the National Academy of Sciences of the United States of America
D R Abbanat, J G Ferry

Abstract

An enzyme complex was isolated from acetate-grown Methanosarcina thermophila that oxidized CO and catalyzed the synthesis or cleavage of acetyl-CoA. The complex consisted of five subunits (alpha1beta1gamma1delta1epsilon1) of 89, 71, 60, 58, and 19 kDa. The complex contained nickel, iron, acid-labile sulfide, and cobalt in a corrinoid cofactor. Two components were resolved by anion-exchange chromatography of the complex in the presence of dodecyltrimethylammonium bromide and Triton X-100: a 200-kDa nickel/iron-sulfur protein with the 89- and 19-kDa (alpha2epsilonx) subunits and a 100-kDa corrinoid/iron-sulfur protein with the 60- and 58-kDa subunits (gamma1delta1). The nickel/iron-sulfur component contained 0.21 Ni, 2.7 Zn, 7.7 Fe, and 13.2 acid-labile sulfide (per alpha1epsilon1). The corrinoid/iron-sulfur component contained 0.7 Co, 0.7 factor III [Coalpha-[alpha-(5-hydroxybenzimidazolyl)]-Cobeta-cyanocobamide], 3.0 Fe, and 2.9 acid-labile sulfide (gamma1delta1). Both components contained iron-sulfur centers. The nickel/iron-sulfur component oxidized CO and reduced methyl viologen or a ferredoxin isolated from M. thermophila. The nickel/iron-sulfur component also oxidized CO and transferred electrons to the corrinoid/iron-sulf...Continue Reading

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Citations

May 16, 2001·Biotechnology and Bioengineering·B W KoonsP J Novak
Jun 8, 2007·Archives of Microbiology·Michael RotherWilliam M Metcalf
May 20, 2005·Molecular Genetics and Genomics : MGG·Raymond HoveyUwe Deppenmeier
Jan 1, 1997·Annual Review of Biochemistry·M L Ludwig, R G Matthews
Oct 7, 2003·Annual Review of Biochemistry·Ruma Banerjee, Stephen W Ragsdale
Oct 26, 2005·Archaea : an International Microbiological Journal·Wonduck KimWilliam B Whitman
Jul 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Weimin GongMichael K Chan
Apr 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·Wei-Qin ZhuangLisa Alvarez-Cohen
Jun 10, 2010·Annual Review of Microbiology·James G Ferry
Jan 1, 1994·Antonie van Leeuwenhoek·M Blaut
Dec 5, 2015·Frontiers in Microbiology·Martijn DienderDiana Z Sousa
Apr 2, 2008·Annals of the New York Academy of Sciences·James G Ferry, Daniel J Lessner
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·S W Ragsdale
Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·J G Ferry
Apr 29, 2016·Microbiology and Molecular Biology Reviews : MMBR·Chris GreeningColin J Jackson
Dec 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·Divya PrakashJames G Ferry
Mar 17, 1999·FEMS Microbiology Reviews·J G Ferry
Mar 21, 2008·Environmental Science & Technology·Eileen B Ekstrom, François M M Morel
Sep 1, 1992·Journal of Bacteriology·J G Ferry
Dec 1, 1996·Journal of Bacteriology·J A Maupin-Furlow, J G Ferry
Aug 1, 1993·Journal of Bacteriology·J D KremerJ Krzycki

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