Resolution of human ribosomal DNA occurs in anaphase, dependent on tankyrase 1, condensin II, and topoisomerase IIα

Genes & Development
Zharko DaniloskiSusan Smith

Abstract

Formation of individualized sister chromatids is essential for their accurate segregation. In budding yeast, while most of the genome segregates at the metaphase to anaphase transition, resolution of the ribosomal DNA (rDNA) repeats is delayed. The timing and mechanism in human cells is unknown. Here we show that resolution of human rDNA occurs in anaphase after the bulk of the genome, dependent on tankyrase 1, condensin II, and topoisomerase IIα. Defective resolution leads to rDNA bridges, rDNA damage, and aneuploidy of an rDNA-containing acrocentric chromosome. Thus, temporal regulation of rDNA segregation is conserved between yeast and man and is essential for genome integrity.

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Citations

Feb 8, 2020·Genes & Development·Kameron Azarm, Susan Smith
Aug 14, 2020·Genes·Alice FinardiRosella Visintin
Nov 2, 2019·Genes·Joyce H Lee, James M Berger
Oct 3, 2020·Biochemical Society Transactions·Erin E Cutts, Alessandro Vannini
Jan 23, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Shannon J McKieAnthony Maxwell
Apr 11, 2021·Seminars in Cell & Developmental Biology·James R PaulsonWilliam C Earnshaw

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