Resolution of the sperm motility-stimulating principle of fowl seminal plasma into Ca2+ and an unidentified low molecular weight factor

Journal of Reproduction and Fertility
K Ashizawa, G J Wishart

Abstract

It was confirmed, using an objective assay of motility, that fowl seminal plasma restores and stimulates the motility of fowl spermatozoa at 40 degrees C in a dose-dependent manner. By separation of a 100,000 g supernatant of fowl seminal plasma with Sephadex G-15, two peaks of motility-stimulating activity were distinguished. One peak coincided with that of calcium and was absent when calcium was removed from the seminal plasma with Dowex 50. The other peak, which accounted for 44% of motility-stimulating activity, contained a low molecular weight, dialysable factor which remains to be identified.

Citations

Jul 31, 2013·Animal Reproduction Science·Margherita MarzoniAntonio Felicioli
Jan 7, 1999·Animal Reproduction Science·L Holm, G J Wishart
Dec 28, 1999·Animal Reproduction Science·G J Wishart, Y I Wilson
Jan 12, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·K AshizawaY Tsuzuki
Jun 1, 1989·British Poultry Science·F van Wambeke, G Huyghebaert
Dec 8, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·K AshizawaY Tsuzuki
Jan 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·K AshizawaY Tsuzuki
Jun 1, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·K AshizawaY Tsuzuki
Jan 1, 1989·Archives of Andrology·C B Lindemann, K S Kanous
Jul 13, 2012·Animal Reproduction Science·Maud BonatoSchalk W P Cloete
Dec 14, 2004·Theriogenology·Véronique DouardElisabeth Blesbois
Jul 15, 2015·PloS One·Ji ZhouBing Yao
Jun 15, 1992·Biochemical and Biophysical Research Communications·K AshizawaY Tsuzuki

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