Resolving and identifying protein components of plant mitochondrial respiratory complexes using three dimensions of gel electrophoresis

Journal of Proteome Research
Etienne H MeyerA Harvey Millar

Abstract

Analyzing highly hydrophobic proteins is a challenge for identification protocols based on gel separation and mass spectrometry. We combined Blue Native and 2D tricine gel electrophoresis to allow separation and identification of respiratory complex subunits from Arabidopsis mitochondria. We identified many of the highly hydrophobic mitochondrion-encoded subunits (GRAVY scores between +0.6 to +1.4) and also found a number of nucleus-encoded proteins associated with complex I for the first time in plants.

References

Mar 1, 1996·The Plant Journal : for Cell and Molecular Biology·L JänschH P Braun
Oct 31, 1996·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·J MarienfeldA Brennicke
Jan 5, 2002·Journal of Bioenergetics and Biomembranes·J Velours, G Arselin
May 15, 2003·Plant Physiology·Joshua L HeazlewoodA Harvey Millar
May 27, 2003·Molecular & Cellular Proteomics : MCP·Myriam FerroNorbert Rolland
Sep 14, 2004·Journal of Proteome Research·Nan ZhangLiang Li
Dec 18, 2004·Plant Molecular Biology·A Harvey MillarHans-Peter Braun
Jan 12, 2005·Trends in Plant Science·A Harvey MillarIan M Møller
Jun 10, 2005·Rapid Communications in Mass Spectrometry : RCM·Robertson CraigRonald C Beavis
Jun 16, 2005·Nature Reviews. Molecular Cell Biology·Bernhard KusterRuedi Aebersold
Aug 6, 2005·Trends in Plant Science·Philip ZimmermannWilhelm Gruissem
Jan 13, 2006·The Journal of Biological Chemistry·Stephanie SunderhausHans-Peter Braun
Aug 1, 2006·Biochimica Et Biophysica Acta·Gabriella PocsfalviSergio Papa
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Joe CarrollJohn E Walker
Jan 2, 2007·Nature Biotechnology·Parag MallickRuedi Aebersold

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Citations

Jun 20, 2008·Molecular Genetics and Genomics : MGG·Claire RemaclePatrice P Hamel
Aug 16, 2011·Plant Physiology·Jennifer KlodmannHans-Peter Braun
Mar 4, 2010·The Plant Cell·Jennifer KlodmannHans-Peter Braun
Feb 22, 2011·Annual Review of Plant Biology·A Harvey MillarDavid A Day
Sep 7, 2011·Biochimica Et Biophysica Acta·Juri DubininFrank Colditz
Oct 18, 2014·Journal of Experimental Botany·Monika W MurchaJames Whelan
Jan 24, 2016·Biochimica Et Biophysica Acta·Nitya SubrahmanianPatrice Paul Hamel
Dec 24, 2015·Frontiers in Plant Science·Marco ZancaniAngelo Vianello
Mar 4, 2011·Phytochemistry·Uma Kota, Michael B Goshe
Dec 21, 2010·Phytochemistry·Jennifer Klodmann, Hans-Peter Braun
Oct 13, 2015·Journal of Proteomics·Puneet PaulEnrico Schleiff
Jun 1, 2011·The Plant Journal : for Cell and Molecular Biology·Kristina KühnJames Whelan
Feb 25, 2014·Mitochondrion·Hans-Peter BraunAnja Zehrmann
Mar 31, 2009·Biochimica Et Biophysica Acta·Martijn A HuynenRadek Szklarczyk
Mar 13, 2015·Plant Signaling & Behavior·Shengchun ZhangChengwei Yang
Oct 18, 2012·Plant Signaling & Behavior·Monika W MurchaJames Whelan
Oct 2, 2013·Biochimica Et Biophysica Acta·Monika W MurchaJames Whelan
Dec 13, 2016·The Plant Journal : for Cell and Molecular Biology·Jennifer SenklerHans-Peter Braun
Sep 10, 2016·Journal of Cell Science·Chris CarrieJürgen Soll
Jan 1, 2008·The Arabidopsis Book·A Harvey MillarJames Whelan
Mar 2, 2019·Annual Review of Plant Biology·Etienne H MeyerChris Carrie
Apr 26, 2020·Mitochondrion·Hans-Peter Braun
Jun 14, 2020·Mitochondrion·Marco ZancaniGiovanna Lippe
Mar 27, 2021·The Plant Cell·Niklas KluschHans-Peter Braun
Nov 3, 2010·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Lihua HanLi-Jia Qu

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