Resolving heterogeneous macromolecular assemblies by Orbitrap-based single-particle charge detection mass spectrometry.

Nature Methods
Tobias P WörnerAlbert J R Heck

Abstract

We demonstrate single-particle charge detection mass spectrometry on an Orbitrap for the analysis of megadalton biomolecular assemblies. We establish that the signal amplitudes of individual ions scale linearly with their charge, which can be used to resolve mixed ion populations, determine charge states and thus also determine the masses of individual ions. This enables the ultrasensitive analysis of heterogeneous protein assemblies including immunoglobulin oligomers, ribosomes, proteinaceous nanocontainers and genome-packed adeno-associated viruses.

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Citations

Aug 21, 2020·Proteomics·Aleksandra A Petelski, Nikolai Slavov
Sep 18, 2020·Molecular & Cellular Proteomics : MCP·Nicholas M RileySharon J Pitteri
Jan 14, 2021·Journal of the American Society for Mass Spectrometry·Anjusha MathewRon M A Heeren
Nov 3, 2020·Journal of Separation Science·Shannon L ThomasKatarína Maráková
Mar 4, 2021·Journal of the American Society for Mass Spectrometry·Gordon RinkeStephan Rauschenbach
Feb 16, 2021·Molecular & Cellular Proteomics : MCP·Nicholas M RileySharon J Pitteri
Apr 20, 2021·Journal of the American Society for Mass Spectrometry·Aleksandr RusinovHiroaki Waki
Apr 23, 2021·Journal of the American Society for Mass Spectrometry·Iain D G Campuzano, Wendy Sandoval
Jun 9, 2021·Nature Methods·Javier Antonio AlfaroChirlmin Joo
Jun 23, 2021·Current Opinion in Biotechnology·Florian FüsslJonathan Bones
Jul 10, 2021·Journal of the American Society for Mass Spectrometry·Tobias NitscheStephen J Blanksby
Dec 5, 2020·Analytical Chemistry·Tobias P WörnerAlbert J R Heck
Oct 30, 2020·Analytical Chemistry·James E KeenerMichael T Marty
Aug 19, 2021·Chemical Reviews·Rivkah Rogawski, Michal Sharon
Aug 21, 2021·Chemical Reviews·Sem TamaraAlbert J R Heck
Aug 28, 2021·Chemical Reviews·Jack L BennettWilliam A Donald
Sep 3, 2021·Chemical Reviews·Amber D Rolland, James S Prell
Oct 28, 2021·Analytical Chemistry·Marius M KostelicMichael T Marty
Nov 3, 2021·Chemical Reviews·Dalton T SnyderVicki H Wysocki

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Methods Mentioned

BETA
glycosylation

Software Mentioned

MSConvert

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