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Resolving microbial microdiversity with high accuracy full length 16S rRNA Illumina sequencing

bioRxiv

Nov 6, 2014

Catherine M Burke, Aaron E. Darling

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Abstract

We describe a method for sequencing full-length 16S rRNA gene amplicons using the high throughput Illumina MiSeq platform. The resulting sequences have about 100-fold higher accuracy than standard Illumina reads and are chimera filtered using information from a single molecule dual tagg...read more

Mentioned in this Paper

RNA, Ribosomal, 16S
Detection
Bacterial 16S RNA
Genes
Chimera Organism
Chimera Disorder
Microbial
Molecular Genetic Technique
Sequencing
Research Methodology
70
59
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  • Resolving microbial microdiversity with high accuracy full length 16S rRNA Illumina sequencing

    bioRxiv

    Nov 6, 2014

    Catherine M Burke, Aaron E. Darling

    PMID: 990010967

    DOI: 10.1101/010967

    Abstract

    We describe a method for sequencing full-length 16S rRNA gene amplicons using the high throughput Illumina MiSeq platform. The resulting sequences have about 100-fold higher accuracy than standard Illumina reads and are chimera filtered using information from a single molecule dual tagg...read more

    Mentioned in this Paper

    RNA, Ribosomal, 16S
    Detection
    Bacterial 16S RNA
    Genes
    Chimera Organism
    70
    59

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