Resolving the Complex Genetic Basis of Phenotypic Variation and Variability of Cellular Growth

Genetics
Naomi ZivDavid Gresham

Abstract

In all organisms, the majority of traits vary continuously between individuals. Explaining the genetic basis of quantitative trait variation requires comprehensively accounting for genetic and nongenetic factors as well as their interactions. The growth of microbial cells can be characterized by a lag duration, an exponential growth phase, and a stationary phase. Parameters that characterize these growth phases can vary among genotypes (phenotypic variation), environmental conditions (phenotypic plasticity), and among isogenic cells in a given environment (phenotypic variability). We used a high-throughput microscopy assay to map genetic loci determining variation in lag duration and exponential growth rate in growth rate-limiting and nonlimiting glucose concentrations, using segregants from a cross of two natural isolates of the budding yeast, Saccharomyces cerevisiae We find that some quantitative trait loci (QTL) are common between traits and environments whereas some are unique, exhibiting gene-by-environment interactions. Furthermore, whereas variation in the central tendency of growth rate or lag duration is explained by many additive loci, differences in phenotypic variability are primarily the result of genetic interact...Continue Reading

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Citations

Aug 12, 2018·Nature Communications·Michael Manhart, Eugene I Shakhnovich
Apr 23, 2019·PLoS Computational Biology·Maria KiskowskiYlenia Chiari
May 6, 2020·Genetics·Jie LinAriel Amir
Aug 18, 2020·PLoS Biology·Kerry A Geiler-SamerotteMark L Siegal
Sep 8, 2020·Journal of Evolutionary Biology·Suda Parimala RavindranMathilde Cordellier
May 12, 2019·Current Genetics·Stephanie Lauer, David Gresham
Nov 27, 2020·Frontiers in Genetics·Jennifer Molinet, Francisco A Cubillos
Feb 16, 2021·Frontiers in Cell and Developmental Biology·Sevan ArabaciyanJean-Pascal Capp

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