Resolving voltage-dependent structural changes of a membrane photoreceptor by surface-enhanced IR difference spectroscopy.

Proceedings of the National Academy of Sciences of the United States of America
X JiangJ Heberle

Abstract

Membrane proteins are molecular machines that transport ions, solutes, or information across the cell membrane. Electrophysiological techniques have unraveled many functional aspects of ion channels but suffer from the lack of structural sensitivity. Here, we present spectroelectrochemical data on vibrational changes of membrane proteins derived from a single monolayer. For the seven-helical transmembrane protein sensory rhodopsin II, structural changes of the protein backbone and the retinal cofactor as well as single ion transfer events are resolved by surface-enhanced IR difference absorption spectroscopy (SEIDAS). Angular changes of bonds versus the membrane normal have been determined because SEIDAS monitors only those vibrations whose dipole moment are oriented perpendicular to the solid surface. The application of negative membrane potentials (DeltaV = -0.3 V) leads to the selective halt of the light-induced proton transfer at the stage of D75, the counter ion of the retinal Schiff base. It is inferred that the voltage raises the energy barrier of this particular proton-transfer reaction, rendering the energy deposited in the retinal by light excitation insufficient for charge transfer to occur. The other structural rear...Continue Reading

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Citations

Aug 5, 2010·Journal of the American Chemical Society·Xiue JiangJoachim Heberle
May 9, 2009·Journal of the American Chemical Society·Ionela RaduJoachim Heberle
Nov 30, 2011·Nano Letters·Ronen AdatoHatice Altug
Dec 5, 2013·Nature Communications·Iban AmenabarRainer Hillenbrand
Feb 22, 2013·Journal of the Royal Society, Interface·Lennart TreuelGerd Ulrich Nienhaus
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Nov 12, 2013·Biochimica Et Biophysica Acta·Víctor A Lórenz-Fonfría, Joachim Heberle
Jul 3, 2013·Biochimica Et Biophysica Acta·Kenichi AtakaJoachim Heberle
Nov 28, 2012·Biochemical Society Transactions·Diego Millo
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Jan 23, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Enrico ForbrigJacek Kozuch
Aug 24, 2017·Chemical Reviews·Jan Philip Kraack, Peter Hamm

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