Resonance assignment and three-dimensional structure determination of a human alpha-defensin, HNP-1, by solid-state NMR.

Journal of Molecular Biology
Yuan ZhangMei Hong

Abstract

Human alpha-defensins [human neutrophil peptides (HNPs)] are immune defense mini-proteins that act by disrupting microbial cell membranes. Elucidating the three-dimensional (3D) structures of HNPs in lipid membranes is important for understanding their mechanisms of action. Using solid-state NMR (SSNMR), we have determined the 3D structure of HNP-1 in a microcrystalline state outside the lipid membrane, which provides benchmarks for structure determination and comparison with the membrane-bound state. From a suite of two-dimensional and 3D magic-angle spinning experiments, (13)C and (15)N chemical shifts that yielded torsion angle constraints were obtained, while inter-residue distances were obtained to restrain the 3D fold. Together, these constraints led to the first high-resolution SSNMR structure of a human defensin. The SSNMR structure has close similarity to the crystal structures of the HNP family, with the exception of the loop region between the first and second beta-strands. The difference, which is partially validated by direct torsion angle measurements of selected loop residues, suggests possible conformational variation and flexibility of this segment of the protein, which may regulate HNP interaction with the pho...Continue Reading

References

Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B L KaganR I Lehrer
Nov 20, 1991·Journal of Molecular Biology·D S WishartF M Richards
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J E GabayC F Nathan
Oct 1, 1985·The Journal of Clinical Investigation·T GanzR I Lehrer
Sep 1, 1994·Protein Science : a Publication of the Protein Society·W C WimleyS H White
Jan 1, 1993·Annual Review of Immunology·R I LehrerT Ganz
Dec 2, 2000·Journal of Biomolecular NMR·D Sharma, K Rajarathnam
Dec 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Aneta T PetkovaRobert Tycko
Feb 5, 2003·Journal of Magnetic Resonance·Charles D SchwietersG Marius Clore
Sep 2, 2003·Nature Reviews. Immunology·Tomas Ganz
Apr 22, 2004·Journal of the American Chemical Society·Tatyana I IgumenovaAnn E McDermott
Aug 20, 2004·The Journal of Peptide Research : Official Journal of the American Peptide Society·Z WuW Lu
Dec 24, 2004·Antimicrobial Agents and Chemotherapy·Bryan EricksenRobert I Lehrer
May 24, 2005·Nature Immunology·Michael E Selsted, Andre J Ouellette
Jun 16, 2005·Journal of the American Chemical Society·Stephan G ZechAnn E McDermott
Feb 9, 2006·Journal of the American Chemical Society·Gaël De PaëpeRobert G Griffin
Apr 29, 2006·Journal of Biomolecular NMR·Gregory J KornhaberGaetano T Montelione
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Rajeswari ManiMei Hong
Nov 8, 2006·Protein Science : a Publication of the Protein Society·Agnieszka SzykJacek Lubkowski
Sep 6, 2007·The Journal of Physical Chemistry. B·Marcos R LourenzoniMaria E D Zaniquelli
Jan 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sarah D Cady, Mei Hong
Mar 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·W Trent FranksChad M Rienstra
Apr 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jonathan J HelmusChristopher P Jaroniec
Jul 31, 2009·The Journal of Biological Chemistry·Gang WeiWuyuan Lu

❮ Previous
Next ❯

Citations

Mar 1, 2012·Journal of Biomolecular NMR·Anders B NielsenBeat H Meier
Apr 24, 2012·Nature Chemistry·Ishita SenguptaChristopher P Jaroniec
Dec 3, 2014·Progress in Nuclear Magnetic Resonance Spectroscopy·Shenlin Wang, Vladimir Ladizhansky
Apr 1, 2014·Journal of the American Chemical Society·Yongchao SuRobert G Griffin
Dec 16, 2011·Immunological Reviews·Robert I Lehrer, Wuyuan Lu
Feb 24, 2011·Protein Science : a Publication of the Protein Society·Mei Hong, Yongchao Su
Aug 31, 2019·Journal of Molecular Modeling·Sheeja V Vasudevan, Ashutosh Kumar
Feb 21, 2021·Advanced Drug Delivery Reviews·Pyae PhyoYongchao Su
May 11, 2020·Microbial Pathogenesis·Alireza NeshaniAbolfazl Jahangiri
May 8, 2013·Journal of the American Chemical Society·Jianping LiJun Yang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.