PMID: 2105212Jan 12, 1990Paper

Resonance energy transfer between points in a reconstituted skeletal muscle thin filament. A conformational change of the thin filament in response to a change in Ca2+ concentration

European Journal of Biochemistry
M Miki

Abstract

The spatial relationships between Lys-61, Cys-374 on actin or SH1 on myosin subfragment-1 (S1) and Cys-190 on tropomyosin or Cys-133 on troponin-I (TnI) in a reconstituted thin filament were studied by fluorescence resonance energy transfer. 5-(2-Iodoacetylaminoethyl)aminonaphthalene 1-sulfonic acid (IAEDANS) attached to Lys-190 on tropomyosin or to Cys-133 on TnI was used as a donor. Fluorescein 5-isothiocyanate (FITC) attached to Lys-61 or 5-(iodoacetoamido)fluorescein (IAF) attached to Cys-374 on actin and 4-dimethylaminophenyl-azophenyl 4'-maleimide (DABMI) attached to SH1 on S1 were used as an acceptor. The transfer efficiency between AEDANS attached to Cys-190 on tropomyosin and FITC attached to Lys-61 on actin was 0.42 in the absence of troponin, 0.46 in the presence of troponin and Ca2+ and 0.55 in the presence of troponin and absence of Ca2+. The corresponding distances between the probes were calculated to be 4.7 nm, 4.6 nm and 4.3 nm respectively, assuming a random orientation factor K2 = 2/3. A large difference in the transfer efficiency from AEDANS attached to Cys-133 on TnI to FITC attached to Lys-61 on actin was observed between in the presence (0.52) and absence (0.70) of Ca2+. The corresponding distances betwee...Continue Reading

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Citations

Jun 15, 1999·Biulleten' eksperimental'noĭ biologii i meditsiny·G V SukoianN V Karsanov
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·D G VassylyevY Maéda
Feb 9, 2010·The Journal of Biological Chemistry·Tomoki AiharaToshiaki Arata
Feb 16, 2005·Annual Review of Physiology·Tomoyoshi Kobayashi, R John Solaro
Feb 21, 2006·Journal of Photochemistry and Photobiology. B, Biology·László MátyusAttila Jenei
Dec 27, 2005·Biochemical and Biophysical Research Communications·Tomoki AiharaToshiaki Arata
Oct 22, 2008·Biophysical Journal·Zoltán UjfalusiMiklós Nyitrai
Feb 3, 2006·Journal of Biochemistry·Hiroaki Mizuno, Hajime Honda
Apr 1, 1992·Journal of Muscle Research and Cell Motility·M MikiC G Dos Remedios
Mar 15, 2006·The Journal of Biological Chemistry·Tomoyoshi Kobayashi, R John Solaro
Jun 1, 1993·Microbiological Reviews·S L Jackson, I B Heath

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