Resonance energy transfer between tryptophan-214 in human serum albumin and acrylodan, prodan, and promen

The Protein Journal
José González-Jiménez, Manuel Cortijo

Abstract

It has been proposed that acrylodan (6-acryloyl-2-dimethylaminonaphthalene) and prodan (6-propionyl-2-dimethylaminonaphthalene) bind to site I of human serum albumin, whereas promen (6-propionyl-2-methoxynaphthalene) binds to site II of this carrier protein. Because human albumin contains only one single tryptophan, at position 214, it has been possible to measure the distances from this amino-acid residue to each of the three probes by nonradiative energy transfer. The distances calculated, 2.97 +/- 0.10 nm, 3.14 +/- 0.11 nm, and 2.62 +/- 0.17 nm, respectively, confirm the locations previously proposed for all three probes.

Citations

May 25, 2007·Journal of Fluorescence·Kwanghee Koh ParkAndrew D Hamilton
Dec 2, 2011·Journal of Photochemistry and Photobiology. B, Biology·E AlarcónE A Lissi
Nov 17, 2006·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Martinus A H CapelleTudor Arvinte
Feb 25, 2015·The Journal of Physical Chemistry. B·Amy M Green, Christopher J Abelt
Oct 2, 2009·Organic & Biomolecular Chemistry·Kwanghee Koh ParkAndrew D Hamilton
May 15, 2019·International Journal of Molecular Sciences·Eszter Fliszár-NyúlMiklós Poór

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