Resonance light scattering method for detecting kanamycin in milk with enhanced sensitivity

Analytical and Bioanalytical Chemistry
Chengke WangDan Chen

Abstract

In this paper, a resonance light scattering (RLS) method was developed for detecting kanamycin with high sensitivity and selectivity. Here, aptamer specific to kanamycin was utilized for recognizing and competitively binding with kanamycin, and gold nanoparticles (GNPs) were used as probes. After adding kanamycin into solutions containing aptamer and GNPs, the GNPs would aggregate in the presence of NaCl, which resulted in the RLS signal intensities enhanced dramatically. Based on this phenomenon, kanamycin can be detected in the range of 10 to 600 nM with a limit of detection as 1 nM, which is more sensitive than many other instrumental methods, especially the commonly used UV-visible spectroscopic method. Furthermore, we demonstrated that this method can be used for detecting kanamycin in milk samples with satisfactory results, which is meaningful for solving food safety problems.

References

Dec 31, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Y H LongJos Hoogmartens
Mar 7, 2007·Analytical Chemistry·Alessandra BiniMarco Mascini
Apr 9, 2008·Journal of Agricultural and Food Chemistry·Yiqiang ChenXilong Xiao
May 4, 2010·Journal of the American Society for Mass Spectrometry·Eun Sun HongSeung Koo Shin
Dec 21, 2010·Biosensors & Bioelectronics·Libing WangChuanlai Xu
May 3, 2011·Analytical Biochemistry·Kyung-Mi SongChangill Ban
Apr 10, 2012·Biosensors & Bioelectronics·Hao FanYuzhi Fang
Feb 1, 2014·Biosensors & Bioelectronics·Xiaojing BaiJilin Tang
Jan 15, 2015·Letters in Applied Microbiology·M Gutiérrez-HuanteJ Sánchez
Nov 4, 2015·Journal of Industrial Microbiology & Biotechnology·C R Strachan, J Davies
Feb 13, 2016·The Analyst·Muslum Ilgu, Marit Nilsen-Hamilton
Feb 18, 2016·Environment International·Hexing WangQingwu Jiang
May 4, 2016·The Science of the Total Environment·Reep Pandi Tasho, Jae Yong Cho

Related Concepts

Photon Correlation Spectroscopy
Metazoa
Bacteriocidal Agents
Food Analysis
Food Contamination
Immunoassay Method
Kantrex
Statistical Sensitivity
Reproducibility of Results
Selex Aptamer Technique

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