Resonance raman analysis of chromophore structure in the lumi-R photoproduct of phytochrome

Biochemistry
F AndelR A Mathies

Abstract

Resonance Raman vibrational spectra of the Pr, lumi-R, and Pfr forms of phytochrome have been obtained using low-temperature trapping and room temperature flow techniques in conjunction with shifted-excitation Raman difference spectroscopy (SERDS). The Pr to lumi-R photoconversion exhibits a thermal barrier and is completely blocked at 30 K, indicating that thermally assisted protein relaxation is necessary for the primary photochemistry. When Pr is converted to lumi-R, new bands appear in the C = C and C = N stretching regions at 1651, 1636, 1590, and 1569 cm-1, indicating that a significant structural change of the chromophore has occurred. The photoconversion also results in an 18 cm-1 decrease in the N-H rocking band in lumi-R. Normal mode calculations correlate this frequency drop with a change in the geometry of the C15 methine bridge of the phytochromobilin chromophore. Additionally, a C = N stretching mode marker band shifts from 1576 cm-1 in Pr to 1569 cm-1 in lumi-R and to 1552 cm-1 in Pfr. Normal mode calculations show that the frequency drop of this band in the lumi-R-->Pfr interconversion is an indication of a C14-C15 syn-->anti conformational change. Moderately intense hydrogen out-of-plane modes that occur at 805...Continue Reading

Citations

Jan 9, 2009·Journal of the American Chemical Society·Jana B NiederRobert Bittl
Jan 5, 2011·The Journal of Physical Chemistry. a·K C TohJohn T M Kennis
Nov 5, 2010·The Journal of Physical Chemistry. B·Alex A SammaMarc Zimmer
Jun 9, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Berthold Borucki
Oct 10, 2006·Physical Chemistry Chemical Physics : PCCP·Bo Durbeej, Leif A Eriksson
May 1, 2008·Physical Chemistry Chemical Physics : PCCP·O Anders Borg, Bo Durbeej
Jan 22, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Angela Strambi, Bo Durbeej
Jan 3, 2006·The Plant Cell·Nathan C Rockwell, J Clark Lagarias
May 4, 2006·Annual Review of Plant Biology·Nathan C RockwellJ Clark Lagarias
Jan 1, 1997·Annual Review of Cell and Developmental Biology·C Fankhauser, J Chory
Sep 26, 2007·Annual Review of Physical Chemistry·Villy Sundström
Feb 4, 2012·The Arabidopsis Book·Jigang LiXing Wang Deng
Jan 5, 2011·The Journal of Physical Chemistry. B·Maria Andrea MroginskiPeter Hildebrandt
Mar 8, 2013·The Journal of Physical Chemistry. B·Heli LehtivuoriJanne A Ihalainen
Nov 23, 2013·The Journal of Physical Chemistry. B·Xuhui ZhuangZhenggang Lan
Jan 19, 2016·Frontiers in Molecular Biosciences·Janne A IhalainenHeli Lehtivuori
Jun 24, 2008·Journal of Molecular Biology·Rei NarikawaMasahiko Ikeuchi
Jul 7, 2007·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Christian SchumannRolf Diller
Mar 5, 2013·Journal of Computational Chemistry·Olle Falklöf, Bo Durbeej
Dec 12, 2012·Photochemistry and Photobiology·Chen SongJörg Matysik
Mar 25, 2014·The Plant Journal : for Cell and Molecular Biology·Altanbadralt SharkhuuChris Gehring
Apr 23, 2015·Nature Chemistry·Francisco Velazquez EscobarPeter Hildebrandt
Sep 30, 2016·Structural Dynamics·Heikki TakalaSebastian Westenhoff
Mar 16, 2018·Chembiochem : a European Journal of Chemical Biology·Uzma ChoudryNigel S Scrutton
Mar 30, 2001·The Journal of Biological Chemistry·C Fankhauser
Jun 29, 2006·The Journal of Biological Chemistry·Katsuhiko InomataTilman Lamparter
Jan 22, 2011·Critical Reviews in Biochemistry and Molecular Biology·Michele E Auldridge, Katrina T Forest
May 13, 2014·The Journal of Biological Chemistry·Anna F E HauckNigel S Scrutton
Aug 3, 2005·The Journal of Biological Chemistry·Berthold BoruckiPeter Hildebrandt
Oct 28, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Heikki TakalaSebastian Westenhoff

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.