Resonance Raman spectroscopy of nitric oxide reductase and cbb(3) heme-copper oxidase

The Journal of Physical Chemistry. B
Eftychia Pinakoulaki, Constantinos Varotsis

Abstract

Elucidating the structure and properties of the active sites in cbb3 heme-copper oxidase and in nitric oxide reductase (Nor) is crucial in understanding the reaction mechanisms of oxygen and nitric oxide reduction by both enzymes. In the work here, we have applied resonance Raman (RR) spectroscopy to investigate the structure and properties of the binuclear heme b3-CuB center of cbb3 heme-copper oxidase from Pseudomonas stutzeri and the dinuclear heme b3-FeB center of Nor from Paracoccus denitrificans in the ligand-free and CO-bound forms and in the reactions with O2 and NO. The RR data demonstrate that in the Nor/NO reaction, the formation of the N-N bond occurs with the His-Fe heme b3 bond intact, and reformation of the heme b3-O-FeB dinuclear center causes the rupture of the proximal His-Fe heme b3 bond. In the reactions of Nor and cbb3 with O2, distinct oxidized heme b3 species, which differ from the as-isolated oxidized forms, have been characterized. The activation and reduction of O2 and NO by cbb3 oxidase and nitric oxide reductase are compared and discussed.

References

Mar 14, 1994·FEBS Letters·M Saraste, J Castresana
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C VarotsisG T Babcock
Jun 12, 1998·Journal of Bioenergetics and Biomembranes·J HendriksM Saraste
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·D A ProshlyakovG T Babcock
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·H Michel
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·A GiuffrèT Soulimane
Jan 15, 2000·The Journal of Biological Chemistry·S HanD L Rousseau
Jul 18, 2001·Journal of the American Chemical Society·T TomitaM Ikeda-Saito
Dec 12, 2001·European Journal of Biochemistry·E ForteA Giuffrè
Feb 5, 2002·The Journal of Biological Chemistry·Eftychia PinakoulakiConstantinos Varotsis
Nov 7, 1996·Chemical Reviews·Richard H. HolmEdward I. Solomon
Nov 7, 1996·Chemical Reviews·Shelagh Ferguson-Miller, Gerald T. Babcock
Apr 25, 2002·The Journal of Biological Chemistry·Eftychia PinakoulakiConstantinos Varotsis
Aug 9, 2002·Journal of the American Chemical Society·Eftychia PinakoulakiConstantinos Varotsis
Mar 15, 2003·The Journal of Biological Chemistry·Eftychia PinakoulakiConstantinos Varotsis
Feb 14, 2007·Angewandte Chemie·Constantinos VarotsisEftychia Pinakoulaki

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Citations

Apr 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ying-Wu LinYi Lu
Apr 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Virve RauhamäkiMårten Wikström
Oct 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·James P CollmanEdward I Solomon
Jul 5, 2015·Biochimica Et Biophysica Acta·Vangelis DaskalakisConstantinos Varotsis
Mar 31, 2015·Physical Chemistry Chemical Physics : PCCP·Takehiro OhtaConstantinos Varotsis
Oct 18, 2011·Biochimica Et Biophysica Acta·Tomoya HinoYoshitsugu Shiro
Aug 23, 2011·The Journal of Physical Chemistry. B·Andrea PavlouEftychia Pinakoulaki
Oct 26, 2016·Biochimica Et Biophysica Acta·Frederic MelinPetra Hellwig
Oct 1, 2016·International Journal of Molecular Sciences·Antonis NicolaidesConstantinos Varotsis
May 12, 2019·FEBS Letters·Maximilian KahlePia Ädelroth
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Célia M SilveiraSmilja Todorovic

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