Respiratory Administration of Infliximab Dry Powder for Local Suppression of Inflammation

AAPS PharmSciTech
Homa FaghihiAlireza Vatanara

Abstract

The airways are verified as a relevant route to improve antibody therapeutic index with superior lung concentration but limited passage into systemic blood stream. The current research aimed to process spray-dried (SD) powder of Infliximab to assess the feasibility of respiratory delivery of antibody for local suppression of lung-secreted tumor necrosis factor α (TNFα). Molecular and structural stability of powders were determined through size exclusion chromatography (SEC-HPLC) and Fourier transform infrared (FTIR) spectroscopy. Particle properties were characterized by laser light scattering, twin stage impinger (TSI), and scanning electron microscopy (SEM). In vitro biological activity was quantified applying L-929 cell line. Ovalbumin (OVA)-challenged balb/c mice were employed to evaluate the anti-TNFα activity of antibody formulation as in vivo experimental model. SD sample consisting of 36 mg trehalose, 12 mg cysteine, and 0.05% of Tween 20 was selected with minimum aggregation/fragmentation rate constants of 0.07 and 0.05 (1/month) based on 1 and 2 months of storage at 40°C and relative humidity of 75%. Fine particle fraction (FPF) value of this formulation was 67.75% with desired particle size and surface morphology for...Continue Reading

References

Aug 1, 1997·Pharmaceutical Development and Technology·Y F MaaC C Hsu
Sep 3, 1999·American Journal of Respiratory and Critical Care Medicine·J V FahyD C Adelman
Dec 12, 2000·The Journal of Allergy and Clinical Immunology·W W BusseS E Wenzel
Mar 27, 2001·Immunology and Cell Biology·H S RamshawA F Lopez
Jan 5, 2002·Pharmaceutical Research·N Y Chew, H K Chan
May 4, 2002·Pharmaceutical Biotechnology·Geoffrey Lee
Mar 31, 2004·International Archives of Allergy and Immunology·Michael LahnWilli K Born
Jun 18, 2004·European Journal of Gastroenterology & Hepatology·Irena KirmanOle H Nielsen
Oct 12, 2004·Immunology and Allergy Clinics of North America·K Suresh BabuStephen T Holgate
Jan 22, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Michael MauryGeoffrey Lee
Jul 15, 2006·American Journal of Respiratory and Critical Care Medicine·Edward M ErinTrevor T Hansel
Dec 5, 2006·International Journal of Pharmaceutics·Fei TianJ Howard Rytting
May 4, 2007·Current Opinion in Pharmacology·Mike BerryAj Wardlaw
Sep 8, 2007·Journal of Pharmaceutical Sciences·David Lechuga-BallesterosMei-Chang Kuo
Nov 27, 2007·The Journal of Allergy and Clinical Immunology·Christopher BrightlingYassine Amrani
Dec 17, 2009·Proceedings of the American Thoracic Society·Emiel F M WoutersJuanita H J Vernooy
Sep 29, 2011·Pharmacology & Therapeutics·Matthew C CatleyKate L Tomlinson
Feb 22, 2012·Lung India : Official Organ of Indian Chest Society·J S Patil, S Sarasija
Mar 20, 2014·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Vahid RamezaniMohammad Seyedabadi
Jun 26, 2014·Biologicals : Journal of the International Association of Biological Standardization·Brian G FeaganAnthony S Russell
May 7, 2015·Biomicrofluidics·Christina Cortez-JugoLeslie Y Yeo
Apr 22, 2016·Molecular and Cellular Pediatrics·Robert C FellnerRobert Tarran
May 14, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Renaud RespaudNathalie Heuzé-Vourc'h
Oct 27, 2017·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Homa FaghihiAlireza Vatanara

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Citations

Oct 30, 2020·Pharmaceutics·Wanling LiangJenny K W Lam
Jun 9, 2020·Advanced Drug Delivery Reviews·Michael Y T ChowHak-Kim Chan
Mar 12, 2021·Advanced Drug Delivery Reviews·Yajie ZhangRobert O Williams
Mar 12, 2021·Advanced Drug Delivery Reviews·Rachel Yoon Kyung ChangHak-Kim Chan
Apr 15, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Eleonore Fröhlich, Sharareh Salar-Behzadi
Sep 2, 2021·AAPS PharmSciTech·Kimberly B ShepardMichael Banks

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Methods Mentioned

BETA
electrophoresis
enzyme-link immunosorbent assay
Light
light scattering
scanning electron microscopy
Bronchoalveolar
ELISA
lavage

Software Mentioned

SPSS
Unicorn
Jasco Spectra Manager
Graph pad prism

Related Concepts

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