Respiratory analysis as a tool to detect physiological changes in Anisakis larvae subjected to stress

Parasitology Research
Isabel Sánchez-AlonsoMercedes Careche

Abstract

Human infection due to eating fish parasitized by live Anisakis larvae in the third stage is considered an important health problem, and the application of treatments to ensure their mortality in the fish products is crucial to prevent the risk of infection. Mobility is used to assess viability, but mobile larvae may not always be infective and immobile larvae may be erroneously considered as non-viable. The objective was to establish whether the analysis of respiratory activity by means of the oxygen consumption rate (OCR) of Anisakis could be used to identify subtle differences between larvae that were still considered viable in terms of their mobility but had been subjected to thermal and/or chemical stress. The metabolic modulators FCCP [carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone] and sodium azide were used and the basal, maximum, spare and residual respiration rates calculated. Results showed that maximum respiratory capacity of larvae subjected to freezing significantly decreased immediately after thawing, but after some acclimatization, they recovered their capacity fully. However, when these larvae were stored at 4.6 °C, their mitochondria became dysfunctional faster than those of untreated larvae. OCR also s...Continue Reading

References

Oct 3, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·E AbolloS D'Amelio
Jan 31, 2006·International Journal for Parasitology·Kyu-Heon KimJoong-Ki Park
Aug 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Alfonso NavasJosé L Martínez
Nov 21, 2007·WormBook : the Online Review of C. Elegans Biology·Bernard Lemire
Apr 11, 2008·Clinical Microbiology Reviews·M Teresa Audicana, Malcolm W Kennedy
Jan 22, 2010·Molecular Biology of the Cell·Iryna O ZubovychMichael G Roth
Jul 6, 2011·The Biochemical Journal·Martin D Brand, David G Nicholls
Oct 26, 2012·International Archives of Allergy and Immunology·M T VenturaR Asero
Jun 29, 2013·The British Journal of Dermatology·M D BrandC L Quinlan
Dec 18, 2013·Advanced Drug Delivery Reviews·Linda P O'ReillyStephen C Pak
Jul 21, 2014·Current Allergy and Asthma Reports·Natalie E Nieuwenhuizen, Andreas L Lopata
Sep 2, 2016·Nature Protocols·Mandy KoopmanRiekelt H Houtkooper
Nov 2, 2016·Canadian Journal of Gastroenterology & Hepatology·Yuto ShimamuraNorman Marcon
Mar 14, 2017·Scientific Reports·Miguel BaoNorval J C Strachan
Apr 22, 2017·Current Allergy and Asthma Reports·Ignacio MoneoMiguel González-Muñoz
Mar 14, 2018·Advances in Parasitology·Simonetta MattiucciGiuseppe Nascetti

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Citations

Apr 15, 2021·Food and Waterborne Parasitology·Isabel Sánchez-AlonsoMercedes Careche

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Methods Mentioned

BETA
chemical treatments
electrophoresis

Software Mentioned

SPSS Statistics

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