PMID: 3033106Apr 1, 1987

Respiratory burst of normal human eosinophils

Journal of Leukocyte Biology
D C PetrecciaR A Clark

Abstract

Oxidative metabolism of eosinophils has generally been studied in cells from patients with eosinophilia. We isolated eosinophils with purity of greater than 95% from normal donors. Oxygen metabolism of eosinophils and neutrophils was compared using O2-. production as a measure of the stimulus-induced respiratory burst. During 10 min of stimulation, using phorbol myristate acetate (PMA) or opsonized zymosan (OPZ), eosinophils produced two to three times as much O2-. as did neutrophils. Continuous kinetic analysis was used to measure the latency period and maximal rate of O2-. production. Eosinophils has a shorter latency period and greater rate of O2-. production than did neutrophils at all concentrations of PMA. With OPZ stimulation, O2-. production rates by both cells were similar. The latency periods were similar with high concentrations of OPZ but at limiting amounts of OPZ eosinophils had a longer latency period than did neutrophils. In addition, the kinetic assay demonstrated that the respiratory burst in eosinophils was more sustained than in neutrophils. These results indicate substantial differences in the oxidative burst of eosinophils and neutrophils with respect to activation, capacity, and regulation. These distinct...Continue Reading

Citations

Apr 18, 1991·The New England Journal of Medicine·P F Weller
Feb 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·P LacyR Moqbel
Feb 27, 2004·Nature·Jatinder AhluwaliaAnthony W Segal
Apr 5, 2018·International Journal of Molecular Sciences·Deok-Hoon KongSukmook Lee
Jun 1, 1989·Scandinavian Journal of Immunology·E MoritaE Christophers
Sep 18, 2001·The Journal of Physiology·Thomas E DecourseyLarry L Thomas
Apr 23, 2003·The Journal of Biological Chemistry·Jeffrey P HendersonJay W Heinecke
Jun 8, 2013·Allergy·G J GleichP F Weller
May 20, 2003·The Journal of Physiology·Deri MorganThomas E DeCoursey
Jan 1, 1997·Memórias do Instituto Oswaldo Cruz·M A Lindsay, M A Giembycz
Oct 2, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ramin LotfiMichael Thomas Lotze
Jan 1, 1988·Annals of the New York Academy of Sciences·R A FromtlingT P Capizzi
Mar 29, 2003·Physiological Reviews·Thomas E Decoursey
Mar 27, 2015·Cellular and Molecular Life Sciences : CMLS·Na LiBevin P Engelward
Jul 18, 2018·Frontiers in Immunology·Linsey PorterEdwin R Chilvers

Related Concepts

Dose-Response Relationship, Drug
Eosinophil
Neutrophil Band Cells
Opsonin
Dioxygen
Superoxides
Tetradecanoylphorbol Acetate, 4a alpha-Isomer
Zymosan

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.