Respiratory carbon metabolism following illumination in intact French bean leaves using (13)C/(12)C isotope labeling

Plant Physiology
Salvador NoguésJaleh Ghashghaie

Abstract

The origin of the carbon atoms in the CO(2) respired by French bean (Phaseolus vulgaris) leaves in the dark has been studied using (13)C/(12)C isotopes as tracers. The stable isotope labeling was achieved through a technical device that uses an open gas-exchange system coupled online to an elemental analyzer and linked to an isotope ratio mass spectrometer. The isotopic analysis of the CO(2) respired in the dark after a light period revealed that the CO(2) was labeled, but the labeling level decreased progressively as the dark period increased. The pattern of disappearance depended on the amount of carbon fixed during the labeling and indicated that there were several pools of respiratory metabolites with distinct turnover rates. We demonstrate that the carbon recently assimilated during photosynthesis accounts for less than 50% of the carbon in the CO(2) lost by dark respiration and that the proportion is not influenced by leaf starvation in darkness before the labeling. Therefore, most of the carbon released by dark respiration after illumination does not come from new photosynthates.

References

Jan 17, 2003·Plant Physiology·Guillaume TcherkezJaleh Ghashghaie
Mar 26, 2003·Journal of Experimental Botany·Carine DevauxPhilippe Raymond

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Citations

Jan 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Guillaume TcherkezGabriel Cornic
Jun 28, 2005·Plant Physiology·Guillaume TcherkezJaleh Ghashghaie
Oct 17, 2008·Plant Physiology·Gary J LaniganUlli Seibt
Feb 27, 2013·Journal of Experimental Botany·Iker AranjueloSalvador Nogués
Aug 7, 2009·The New Phytologist·Ulrike GamnitzerHans Schnyder
Aug 17, 2010·Journal of Plant Physiology·Alvaro Sanz-SáezManuel Sánchez-Díaz
Jan 9, 2008·The New Phytologist·David R BowlingJames T Randerson
Jul 10, 2007·Plant, Cell & Environment·Guillaume TcherkezHoward Griffiths
Nov 4, 2006·Plant, Cell & Environment·James L PraterJeffrey P Chanton
Mar 10, 2007·Rapid Communications in Mass Spectrometry : RCM·Christiane WernerCristina Máguas
Nov 28, 2007·Rapid Communications in Mass Spectrometry : RCM·Elena HettmannGerd Gleixner
Dec 17, 2008·Rapid Communications in Mass Spectrometry : RCM·Antoni PardoSalvador Nogués
May 10, 2005·Rapid Communications in Mass Spectrometry : RCM·Franz-W BadeckJaleh Ghashghaie
Jul 16, 2009·Rapid Communications in Mass Spectrometry : RCM·Christiane WernerPierrick Priault
Jul 16, 2009·Rapid Communications in Mass Spectrometry : RCM·Florence Maunoury-DangerGuillaume Tcherkez
Aug 5, 2009·Rapid Communications in Mass Spectrometry : RCM·Iker AranjueloSalvador Nogués
Jan 20, 2010·Plant, Cell & Environment·Guillaume TcherkezJaleh Ghashghaie
Dec 14, 2005·Rapid Communications in Mass Spectrometry : RCM·Salvador NoguésJaleh Ghashghaie
Jun 20, 2012·Plant, Cell & Environment·Paul P G GauthierGuillaume Tcherkez
Feb 1, 2011·Plant, Cell & Environment·Margaret M BarbourHamish Brown
Jun 1, 2008·Functional Plant Biology : FPB·Iker AranjueloSalvador Nogués
Jun 1, 2012·Plant, Cell & Environment·Guillaume TcherkezRichard Bligny

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