Respiratory Multiplex Polymerase Chain Reaction: An Important Diagnostic Tool in Immunocompromised Patients

Indian Journal of Critical Care Medicine : Peer-reviewed, Official Publication of Indian Society of Critical Care Medicine
Amarjeet KaurYatin Mehta

Abstract

Viruses and atypical pathogens can cause significant respiratory illness in immunocompromised patients. Multiplex polymerase chain reaction (MPCR) has improved the diagnostic yield of pathogens, and it is easier to identify the co-infections also. The present study was done to evaluate the performance of MPCR on bronchoalveolar lavage (BAL) samples in immunocompromised patients. Atotal of 177 BAL specimens collected over a 19 months period from immunocompromised patients with respiratory illness were analyzed with the MPCR and aerobic culture. Patients were divided into four according to the pathogens. Category V (only viral), Category NV (nonviral, i.e., bacteria and atypical), Category M (mixed, i.e., both viral and nonviral pathogen), and Category UK (unknown etiology). MPCR identified the causative pathogen in 59.3% of patients while culture could identify only in 37.8% of patients. Most frequent etiological agent was Klebsiella pneumoniae (32%), followed by cytomegalovirus (21%), and Pneumocystis jirovecii (10%). Numbers of patients in each category were Category V (9.6%), Category NV (43.5%), Category M (19.8%), and Category UK (27.1%). Mortality was significantly higher in patients of Category M having mixed infections. ...Continue Reading

References

Aug 1, 1982·American Journal of Surgery·J Sanguily
Apr 15, 1999·Respiration; International Review of Thoracic Diseases·R P Baughman
May 27, 1999·Respiration Physiology·H H MeissnerA J Williams
May 31, 2002·Journal of Clinical Microbiology·Cristina DanésJorge Puig de la Bellacasa
Feb 15, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Roy F ChemalyRobin K Avery
Jul 12, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kate E TempletonEric C J Claas
Nov 3, 2005·Clinics in Chest Medicine·Todd D Barton, Emily A Blumberg
Feb 6, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Lionel A MandellUNKNOWN American Thoracic Society
Jul 20, 2007·Journal of General Internal Medicine·Ellen F T YeeRichard M Hoffman
Oct 16, 2007·Chest·Guillaume BolléeElie Azoulay
Feb 29, 2008·Critical Care : the Official Journal of the Critical Care Forum·Xavier MonnetChristian Richard
Mar 12, 2009·Current Opinion in Pulmonary Medicine·Marcelo CortiKumiko Eiguchi
Mar 12, 2009·Current Opinion in Infectious Diseases·Maria Angeles MarcosAntoni Torres
Apr 28, 2009·European Journal of Internal Medicine·Abdul Rahman Safadi, Ayman O Soubani
May 22, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·L RenJ Wang
Oct 28, 2009·Respirology : Official Journal of the Asian Pacific Society of Respirology·UNKNOWN Japanese Respiratory Society
Jan 13, 2010·Clinical Microbiology Reviews·John S Tregoning, Jürgen Schwarze
Jan 19, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Robin Brittain-LongLars-Magnus Andersson
Jun 8, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P M SoccalL Kaiser
Sep 15, 2010·Journal of Intensive Care Medicine·Karen Joan VigilRoy F Chemaly
Oct 14, 2010·Dimensions of Critical Care Nursing : DCCN·Patricia JuarezCheryl A Lefaiver
Apr 7, 2011·Scandinavian Journal of Infectious Diseases·Niclas JohanssonJonas Hedlund
Apr 28, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·D-L VuL Kaiser
Sep 17, 2011·The European Respiratory Journal·K MühlethalerC Garzoni
Nov 11, 2011·Frontiers in Pharmacology·Alejandra López-GiraldoAntoni Torres
May 19, 2012·Clinical Radiology·S L JepsonV Raj
Jun 16, 2012·American Journal of Respiratory and Critical Care Medicine·Sang-Ho ChoiYounsuck Koh
Oct 16, 2012·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Ming-Chi LiWen-Chien Ko
Aug 19, 2014·Pneumonologia i alergologia polska·Mateusz Marek PolaczekKazimierz Roszkowski-Śliż

❮ Previous
Next ❯

Methods Mentioned

BETA
nucleic acid amplification
bronchoalveolar lavage
nucleic acid extraction
bronchoalveolar
lavage
biopsy
PCR

Software Mentioned

Statistical Package for the Social Sciences system SPSS Statis...

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.