Respiratory syncytial virus infection accelerates lung fibrosis through the unfolded protein response in a bleomycin-induced pulmonary fibrosis animal model

Molecular Medicine Reports
Lina WangZhimin Zhang

Abstract

Emerging evidence has demonstrated that endoplasmic reticulum stress (ER) is involved in the pathogenesis of idiopathic pulmonary fibrosis, however, the underlying mechanism remains unclear. Viral infection often triggers a hyperinflammatory response by an expansion of the ER. The present study was designed to observe the role of respiratory syncytial virus infection (RSV)‑induced ER stress on lung fibrosis. In order to determine the role of ER stress on the onset and progression of pulmonary fibrosis, mice received an intratracheal combined injection of RSV and bleomycin on day 0. At day 7, 14 and 21 following combined injection, RSV in the lung tissues was assayed by immunohistochemistry, cellular classification was assayed by direct microscopic observation after Wright staining and the secretion of cytokines in the broncho‑alveolar lavage fluid (BALF) was assayed by ELISA. The expression of collagen type I was assayed by immunofluorescence and western blot analysis. The expression of ER stress related proteins was analyzed by western blot. In addition, the correlations of ER‑stress related proteins with collagen type‑1 were examined. RSV administration resulted in increased inflammation, as demonstrated by increased levels o...Continue Reading

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Citations

Jun 22, 2018·Clinical & Translational Immunology·Alice C-H ChenMichael A McGuckin
May 25, 2021·Frontiers in Medicine·Mirjam KienerMarianna Kruithof-de Julio
Oct 14, 2021·Microbiology and Molecular Biology Reviews : MMBR·Evelyne KohliCarmen Garrido
Oct 28, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kempaiah RayavaraYashoda M Hosakote
Aug 16, 2019·Respiration; International Review of Thoracic Diseases·Min SongYong Zhou

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