PMID: 9557748Apr 29, 1998Paper

Respiratory syncytial virus infection of human alveolar epithelial cells enhances interferon regulatory factor 1 and interleukin-1beta-converting enzyme gene expression but does not cause apoptosis

Journal of Virology
R TakeuchiS Chiba

Abstract

The induction kinetics of the transcriptional activities of interferon regulatory factor 1 (IRF-1), interleukin-1beta-converting enzyme (ICE), and CPP32 by respiratory syncytial virus (RSV) infection of human type II alveolar epithelial cells (A549 cells) were analyzed semiquantitatively by reverse transcriptase PCR. The appearance of ICE and CPP32 protein in cell lysate was examined by Western blotting analysis. The induction of apoptosis by RSV infection was examined by the appearance of DNA fragmentation detected by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling. RSV moderately enhanced IRF-1 mRNA as early as 4 h after infection, and this enhancement lasted several hours. Following induction of the IRF-1 gene, ICE gene expression increased significantly, and an increase of ICE protein was observed in the RSV-infected cell lysate. These increments were observed in cells treated with live RSV but not in cells treated with inactivated RSV or control antigen. However, no infection-specific increase of CPP32 gene expression or the protein was observed. No nucleosomal fragmentation was observed in RSV-infected cells during the whole course of infection, despite the appearance of extensive cytopathic ch...Continue Reading

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Citations

Sep 19, 2003·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Santanu Bose, Amiya K Banerjee
Nov 26, 2005·Uirusu·Hiroyuki Tsutsumi
Feb 27, 2014·BMB Reports·Tae Hoon Kim, Heung Kyu Lee
Jan 25, 2013·Viruses·Elske van den BergReinout A Bem
Sep 18, 2010·Nature Reviews. Immunology·Thirumala-Devi Kanneganti
Dec 19, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M BehrC Jungwirth
Jun 26, 2001·Scandinavian Journal of Immunology·J Pirhonen
May 20, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·April KalinowskiJonathan L Koff
Nov 5, 1999·Annual Review of Microbiology·A RoulstonP E Branton
Oct 19, 1999·Gene·Y MamaneJ Hiscott

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