PMID: 9420296Jan 7, 1998Paper

Respiratory syncytial virus infection of neonatal monocytes stimulates synthesis of interferon regulatory factor 1 and interleukin-1beta (IL-1beta)-converting enzyme and secretion of IL-1beta

Journal of Virology
R TakeuchiS Chiba

Abstract

Interleukin-1beta (IL-1beta) production in response to respiratory syncytial virus (RSV) was investigated in normal neonate monocytes. Intracellular or culture supernatant IL-1beta protein levels were measured by enzyme immunoassay. The expression of mRNAs for interferon regulatory factor 1 (IRF-1), IL-1beta-converting enzyme (ICE), and IL-1beta in the cells was analyzed semiquantitatively by reverse transcriptase-PCR. Before RSV exposure, some IRF-1, ICE, and IL-1beta transcripts were already expressed in the monocytes. The levels of these transcripts increased significantly 2 h after RSV exposure compared with those in mock-infected cells. At that time, significantly higher intracellular IL-1beta protein levels were observed in RSV-exposed cells. After 20 h of RSV exposure, quantities of soluble IL-1beta secreted from RSV-exposed cells were moderately higher than those from noninfected cells. These observations suggest that RSV infection of neonatal monocytes triggers enhanced transcription and increased translation of the IL-1beta gene and increased secretion of the soluble protein. The later phase of these processes may be promoted by ICE activity, which was upregulated by increased IRF-1. The increase in IRF-1 activity may...Continue Reading

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Citations

Mar 20, 2001·American Journal of Respiratory and Critical Care Medicine·J L Kimpen
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joann Y RichardsonJorge C G Blanco
Aug 12, 2017·International Journal of Molecular Sciences·Nicolás M S GálvezAlexis M Kalergis
Dec 17, 2014·Virulence·Janyra A EspinozaAlexis M Kalergis
Oct 19, 1999·Gene·Y MamaneJ Hiscott
Jul 3, 2002·Virology·Kathleen Azzam-SmoakBarbara Sherry
Mar 7, 2003·Microbial Pathogenesis·Jun YangDeborah F Talkington
Jul 25, 2000·Clinics in Chest Medicine·A TuffahaR F Lemanske
Jul 25, 2003·Pediatric Clinics of North America·James E Gern, Robert F Lemanske

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