Respiratory syncytial virus persistence in macrophages downregulates intercellular adhesion molecule-1 expression and reduces adhesion of non-typeable Haemophilus influenzae

Intervirology
Gerardo ArrevillagaBeatríz Gómez

Abstract

Persistence of respiratory syncytial virus (RSV) has been associated with episodes of chronic obstructive pulmonary disease (COPD); furthermore, co-infection of RSV with non-typeable Haemophilus influenzae (NTHi) is increasingly recognized as a cause of exacerbations of COPD. To study whether RSV persistence in a macrophage (Mφ)-like cell line alters NTHi uptake (adhesion and ingestion). A murine Mφ-like cell line persistently infected with RSV (MφP) was used. The effects of RSV persistence on NTHi uptake by MφP and mock-infected Mφ (MφN) were determined by flow-cytometric assays with NTHi labelled with either ethidium bromide or FITC. Expression of intercellular adhesion molecule-1 (ICAM-1), a ligand for NTHi, was determined by measuring mRNA through real-time PCR and protein by Western blot assays. RSV persistence reduced both the capacity of Mφ to take up bacteria and the expression of ICAM-1 mRNA and protein. Furthermore, when ICAM-1 was blocked with anti-ICAM-1 antibody, the adhesion capacity of NTHi was significantly reduced for MφN, whereas for MφP the effect was less evident, implying that ICAM-1 participates in NTHi adhesion to Mφ. RSV persistence in murine Mφ diminishes their capacity to adhere and ingest NTHi through...Continue Reading

References

Jul 1, 1990·The Journal of Clinical Investigation·J R PanuskaY T Huang
Apr 1, 1987·Infection and Immunity·A A CampagnariM A Apicella
Mar 8, 2000·The European Respiratory Journal·S Z WangK D Forsyth
Feb 28, 2002·Annual Review of Immunology·David M Underhill, Adrian Ozinsky
Mar 20, 2002·Virus Research·Rosa Elena SarmientoBeatríz Gómez
May 15, 2002·International Journal of Antimicrobial Agents·Alistair Leanord, Craig Williams
Apr 12, 2003·American Journal of Respiratory Cell and Molecular Biology·Irini Lazou AhrénKristian Riesbeck
Jan 27, 2004·American Journal of Respiratory and Critical Care Medicine·Jurgen SchwarzePeter J M Openshaw
Nov 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Akira YamauchiMary C Dinauer
May 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ralf Arnold, Wolfgang König
Feb 4, 2006·American Journal of Respiratory and Critical Care Medicine·Tom M A WilkinsonJadwiga A Wedzicha
Mar 21, 2007·Current Opinion in Pharmacology·Suzanne L Traves, David Proud
Dec 14, 2007·Experimental Lung Research·Helen M Marriott, David H Dockrell
Jun 3, 2008·The European Respiratory Journal·K F Chung, I M Adcock
Jul 16, 2008·Clinical Microbiology Reviews·Shyam S Mohapatra, Sandhya Boyapalle
Oct 23, 2008·The Pediatric Infectious Disease Journal·Asunción MejíasOctavio Ramilo
Oct 23, 2008·The Pediatric Infectious Disease Journal·Markus B SikkelSebastian L Johnston
Oct 3, 2008·The Journal of Infectious Diseases·Dora EstripeautAsuncion Mejias
Aug 4, 2009·PLoS Pathogens·Michael B A Oldstone
Sep 11, 2009·Proceedings of the American Thoracic Society·Sanjay SethiSebastian L Johnston
Nov 10, 2009·The European Respiratory Journal·A E TaylorL E Donnelly

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Citations

Apr 5, 2014·Current Opinion in Infectious Diseases·Farshid Jalalvand, Kristian Riesbeck
Dec 3, 2016·The Journal of General Virology·Carla BellinghausenFrank R M Stassen
Feb 28, 2018·Innate Immunity·Nikita H TrivediBernard P Arulanandam
Nov 20, 2019·Journal of Clinical Medicine·Konstantinos LoverdosNikoletta Rovina

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