Response kinetics of radiation-induced micronucleated reticulocytes in human bone marrow culture

Mutation Research
Hongliang SunYuhchyau Chen

Abstract

The frequency of micronucleated reticulocytes (MN-RETs) in the bone marrow or peripheral blood is a sensitive indicator of cytogenetic damage. While the kinetics of MN-RET induction in rodent models following irradiation has been investigated and reported, information about MN-RET induction of human bone marrow after radiation exposure is sparse. In this report, we describe a human long-term bone marrow culture (LTBMC), established in three-dimensional (3D) bioreactors, which sustains long-term erythropoiesis. Using this system, we measured the kinetics of human bone marrow red blood cell (RBC) and reticulocyte (RET) production, as well as the kinetics of human MN-RET induction following radiation exposure up to 6Gy. Human bone marrow established in the 3D bioreactor demonstrated an average percentage of RBCs among total viable cells peaking at 21% on day 21. The average percentage of RETs among total viable cells reached a maximum of 11% on day 14, and remained above 5% by day 28, suggesting that terminal erythroid differentiation was still active. Time- and dose-dependent induction of MN-RET by gamma radiation was observed in the human 3D LTBMC, with peak values occurring at approximately 3 days following 1Gy irradiation. A t...Continue Reading

References

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Citations

Mar 20, 2016·Tissue Engineering. Part C, Methods·Yu-Suke TorisawaDonald Ingber
Jan 27, 2015·Annual Review of Pathology·Kambez H BenamDonald E Ingber
May 28, 2019·Radiation and Environmental Biophysics·Małgorzata M DobrzyńskaJoanna Radzikowska

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