PMID: 11329615May 1, 2001Paper

Response of alkalinization or acidification by phytohemagglutinin is dependent on the activity of protein kinase C in human peripheral T Cells

Journal of Cellular Biochemistry
E J ChienJ E Wang

Abstract

The increase of intracellular free calcium concentration ([Ca(2+)](i)) and protein kinase C (PKC) activity are two major early mitogenic signals to initiate proliferation of human T cells. However, a rapid change in intracellular pH (pH(i)), acidification or alkalinization during the activation, is also associated after these two signals. The aim of this study was to define whether the change in pH(i) is affected by calcium and protein kinase C (PKC), in phytohemagglutinin (PHA)-stimulated T cells. T cells were isolated from human peripheral blood. The [Ca(2+)](i) and the pH(i) were measured using, respectively, the fluorescent dyes, Fura-2, and BCECF. In addition, down-regulation of PKC activity by PMA (1 microM, 18 h) was confirmed in these cells using a protein kinase assay. The results indicated that, (1) alkalinization was induced by PHA or PMA in T cells; the results of alkalinization was PKC-dependent and Ca(2+)-independent, (2) in PKC down-regulated T cells, PHA induced acidification; this effect was enhanced by pre-treating the cells with the Na(+)/H(+) exchange inhibitor, 5-(N,N-dimethyl)-amiloride, (DMA, 10 microM, 20 min), (3) the acidification was dependent on the Ca(2+) influx and blocked by removal of extracellul...Continue Reading

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Citations

Aug 3, 2010·The Journal of Biological Chemistry·Gillian L DruryImed-Eddine Gallouzi
Dec 4, 2003·British Journal of Pharmacology·Virginie AiresNaim Akhtar Khan
Jun 1, 2006·American Journal of Physiology. Renal Physiology·Wan-Young KimJin Kim
Feb 27, 2007·Journal of Cellular Physiology·Eileen Jea ChienMing-Ta Hsu

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