Response of Bdellovibrio and like organisms (BALOs) to the migration of naturally occurring bacteria to chemoattractants

Current Microbiology
Ashvini Chauhan, Henry N Williams

Abstract

A dual culture-based and non-culture-based approach was applied to characterize predator bacterial groups in surface water samples collected from Apalachicola Bay, Florida. Chemotaxis drop assays were performed on concentrated samples in an effort to isolate predator bacteria by their chemotactic ability. Yeast extract (YE) and casamino acids (CA) proved to be strong chemoattractants and resulted in three visibly distinct bands; however, dextrose, succinate, pyruvate, and concentrated cells of Vibrio parahaemolyticus P5 as prey did not elicit any response. The three distinct bands from YE and CA were separately collected to identify the chemotactic microbial assemblages. Plaque-forming unit assays from different chemotaxis bands with P5 as prey indicated 5- (CA) to 10-fold (YE) higher numbers of predator bacteria in the outermost chemotactic bands. Polymerase chain reaction-restriction fragment length polymorphism and 16S rDNA sequencing of clones from different chemotaxis bands resulted in identification of Pseudoalteromonas spp., Marinomonas spp., and Vibrio spp., with their numbers inversely proportional to the numbers of predators-i.e., Bdellovibrio spp. and Bacteriovorax spp-in the chemotaxis bands. This study indicates th...Continue Reading

References

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Citations

Mar 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ashvini ChauhanHenry N Williams
Mar 31, 2012·PloS One·Huan ChenHenry N Williams
Feb 19, 2009·Research in Microbiology·Adrian A Medina, Daniel E Kadouri
Oct 22, 2008·Advances in Microbial Physiology·Carey LambertLiz Sockett
Dec 5, 2016·FEMS Microbiology Ecology·Sally OttoLukas Y Wick
Sep 5, 2020·The ISME Journal·Rajesh SathyamoorthyEdouard Jurkevitch
Mar 19, 2021·FEMS Microbiology Ecology·Rajesh SathyamoorthyEdouard Jurkevitch

Related Concepts

Bdellovibrio
Macrophage Chemotactic Factors
Chemotaxis
DNA, Ribosomal
Bionomics
Phylogeny
Restriction Fragment Length Polymorphism
RNA, Ribosomal, 16S
Seawater
Streak Plate Count

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