Abstract
We investigated the suitability of pools of overlapping synthetic peptides spanning the complete alpha 1 subunit sequence of the human muscle acetylcholine receptor (AChR) (alpha 1 pool) or the extracellular domain (residues 1-218, alpha 11-218 pool), and of biosynthetic alpha 1 constructs from E. coli, as stimulants of human CD4+ cells from myasthenia gravis (MG) patients and healthy subjects. A construct corresponding to residues alpha 11-209 was obtained as solubilized inclusion bodies (ib alpha 11-209), or purified by SDS gel electrophoresis (pur alpha 11-209). A second construct included the extracellular, cytoplasmic and carboxylterminal domains plus histidine residues, and was obtained as inclusion bodies (ib alpha 1NoTrans) or purified by gel permeation and histidine tag affinity chromatography (pur alpha 1NoTrans). A biosynthetic extracellular domain of the neuronal AChR alpha 7 subunit (ib alpha 71-206) isolated from E. coli as inclusion bodies served as control for bacterial contaminants. We used ib alpha 11-209, pur alpha 11-209 and peptide pools to propagate CD4+ lines from two MG patients. The lines obtained using pur alpha 11-209 and the peptide pools recognized the peptide pools and alpha 1 constructs tested wel...Continue Reading
References
May 11, 1992·Neurology·A A ManfrediB M Conti-Tronconi
Jul 9, 1992·Nature·P V LehmannE E Sercarz
Oct 1, 1992·Immunology Today·S Romagnani
Sep 1, 1992·Clinical Immunology and Immunopathology·R OrE W Gelfand
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R A Houghten
Jun 1, 1989·Journal of Autoimmunity·J Newsom-DavisJ B Rothbard
Dec 1, 1988·Brain, Behavior, and Immunity·A Vincent
Jan 4, 1988·FEBS Letters·R SchoepferJ Lindstrom
Aug 15, 1970·Nature·U K Laemmli
Sep 28, 1993·Biochemistry·R AnandJ Lindstrom
Oct 1, 1993·Current Opinion in Biotechnology·V A Luckow
Jan 1, 1994·Critical Reviews in Biochemistry and Molecular Biology·B M Conti-TronconiM P Protti
Apr 1, 1993·The Journal of Experimental Medicine·R ManettiS Romagnani
Jul 1, 1993·Immunology Today·G Trinchieri
Mar 17, 1994·Nature·L J SternD C Wiley
Jun 23, 1994·The New England Journal of Medicine·D B Drachman
Oct 1, 1996·Current Opinion in Biotechnology·I Jones, Y Morikawa
Jun 1, 1997·Immunology Today·S Romagnani
Jun 1, 1997·Neurology·Z Y WangB M Conti-Fine
Dec 31, 1997·Journal of Neuroimmunology·R VoltzK Dornmair
Citations
Mar 1, 2006·Expert Review of Clinical Immunology·Bethan Lang, Nick Willcox
Jun 24, 2008·Annals of the New York Academy of Sciences·Nick WillcoxAngela Vincent
Jun 29, 2004·Human Immunology·Molly DayanEdna Mozes
Dec 24, 2013·Retrovirology·Annie BernierPetronela Ancuta
Mar 15, 2000·Muscle & Nerve·J M Lindstrom