Response of Corynebacterium glutamicum exposed to oscillating cultivation conditions in a two- and a novel three-compartment scale-down bioreactor

Biotechnology and Bioengineering
Anja LemoineStefan Junne

Abstract

The oscillatory conditions in substrate and oxygen supply that typically occur on a large (industrial) scale are usually simulated in two-compartment scale-down reactors. In this study, the performance of nutrient-limited fed-batch cultivations of Corynebacterium glutamicum in a standard two-compartment reactor (two-CR) is compared to the performance in a novel three-compartment reactor (three-CR). The three-CR is designed to mimic three distinct zones of an industrial scale bioreactor that occur if the feed addition is installed at the bottom of the fluid phase. Our findings show that lactate and succinate appear in concentrations two-fold higher in the three-CR cultivation than in the two-CR cultivation. Similar results are revealed for the amino acids glycine, threonine, glutamate, and glutamine. In contrast to the two-CR cultivation, no intracellular accumulation of pyruvate is observed in the three-CR cultivation, since the carbon fluxes are directed toward lactate. As previously reported, the expression of lactate dehydrogenase (LDH) is increased in the context of oxygen deprivation. Thus, C. glutamicum adapts to the oscillating environment in the three-CR. This successful adaptation is revealed by a flow cytometric analy...Continue Reading

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Citations

Aug 11, 2016·Nucleic Acids Research·Irem Avcilar-KucukgozeZoya Ignatova
Jun 25, 2017·Metabolic Engineering·Dennis BinderAlexander Grünberger
Mar 11, 2018·Applied Microbiology and Biotechnology·Howard Ramirez-MaluleRigoberto Ríos-Estepa
Mar 10, 2019·Biotechnology Progress·Marius ConradyStefan Junne
Sep 24, 2019·Critical Reviews in Biotechnology·Fernanda Karine do Carmo FélixCarlos Ricardo Soccol
Dec 10, 2019·Biotechnology and Bioengineering·Guan WangSiliang Zhang
Apr 28, 2017·Microbial Biotechnology·Joana Danica SimenUNKNOWN RecogNice-Team
Dec 20, 2018·Frontiers in Bioengineering and Biotechnology·Klaus Pellicer-AlborchStefan Junne
Mar 16, 2018·Bioprocess and Biosystems Engineering·Anna-Lena Heins, Dirk Weuster-Botz
Nov 11, 2016·Engineering in Life Sciences·Julian LangeBastian Blombach

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Bioreactors (ASM)

Bioreactors are important devices or systems that utilize living cells or enzymes for a chemical process. These devices carefully control and monitor the environment factors such as pH, nutrients level, and temperature, which can affect the yield in a given process. Discover the latest research here.