Response of digestive enzymes and esterases of ecotoxicological concern in earthworms exposed to chlorpyrifos-treated soils

Ecotoxicology
Juan C Sanchez-HernandezA M Attademo

Abstract

Assessment of organophosphorus (OP) pesticide exposure in non-target organisms rarely involves non-neural molecular targets. Here we performed a 30-d microcosm experiment with Lumbricus terrestris to determine whether the activity of digestive enzymes (phosphatase, β-glucosidase, carboxylesterase and lipase) was sensitive to chlorpyrifos (5 mg kg-1 wet soil). Likewise, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities were measured in the wall muscle and gastrointestinal tissues as indicators of OP exposure. Chlorpyrifos inhibited the acid phosphatase (34% of controls), carboxylesterase (25.6%) and lipase activities (31%) in the gastrointestinal content. However, in the gastrointestinal tissue, only the carboxylesterase and lipase activities were significantly depressed (42-67% carboxylesterase inhibition in the foregut and crop/gizzard, and 15% lipase inhibition in the foregut). Chlorpyrifos inhibited the activity of both cholinesterases in the gastrointestinal tissues, whereas the AChE activity was affected in the wall muscle. These results suggested chlorpyrifos was widely distributed throughout the earthworm body after 30 d of incubation. Interestingly, we found muscle carboxylesterase activity strongl...Continue Reading

References

Jul 1, 1990·Environmental Health Perspectives·T R Fukuto
Nov 24, 1999·Journal of Biochemical and Molecular Toxicology·G B Quistad, J E Casida
Nov 21, 2002·Analytical Biochemistry·Namita GuptaRani Gupta
Jan 18, 2003·Analytical Biochemistry·Peter EyerElsa Reiner
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE
Jun 1, 2005·Ecotoxicology and Environmental Safety·J RömbkeW Didden
Feb 2, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Gary B QuistadJohn E Casida
Mar 10, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Magali RaultYvan Capowiez
May 18, 2007·Annual Review of Microbiology·Harold L Drake, Marcus A Horn
Dec 23, 2008·Nature Reviews. Microbiology·Brajesh K Singh
Dec 17, 2009·Archives of Biochemistry and Biophysics·Patrick Masson, Oksana Lockridge
Feb 4, 2010·Analytical and Bioanalytical Chemistry·M Asensio-RamosM A Rodríguez-Delgado
Nov 3, 2010·Journal of Environmental Management·Neeru KadianPrem Dureja
Jul 7, 2014·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Juan Manuel RíosPablo Sabat
Oct 19, 2016·Environmental Toxicology·Ptumporn MuangphraRavi Gooneratne

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
Sandra González VejaresJuan C Sanchez-Hernandez
The Science of the Total Environment
Juan C Sanchez-HernandezMagali Rault
Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
Juan C Sanchez-HernandezMagali Rault
© 2022 Meta ULC. All rights reserved