Response of embryo axes of germinating seeds of yellow lupine to Fusarium oxysporum

Plant Physiology and Biochemistry : PPB
Iwona MorkunasMonika Kozłowska

Abstract

Defence responses of embryo axes of Lupinus luteus L. cv. Polo were studied 48-96 h after inoculation with Fusarium oxysporum Schlecht f.sp. lupini. The infection restricted the growth of embryo axes, the lengths of infected embryo axes 72 and 96 h after inoculation were 11 and 12 mm less in the controls, respectively, while their masses c. 0.03 g less than in the controls. The concentration of H2O2 in embryo axes of inoculated germinating seeds was higher than in the control. This was probably a consequence of oxidative burst as well as H2O2 generation by the invading necrotrophic fungal pathogen. EPR-based analyses detected the presence of free radicals with g1 and g2 values of 2.0052 +/- 0.0004 and 2.0031 +/- 0.0005, respectively. Concentrations of the radicals 72 and 96 h after inoculation were 50% higher than in the control. The values of the spectroscopic splitting coefficients suggest that they are quinone radicals. However, inoculated embryo axes possess a number of adaptive mechanisms protecting them from oxidative damage. A twofold increase in catalase (CAT, EC 1.11.1.6) activity was evidenced in embryo axes infected with F. oxysporum Schlecht f. sp. lupini, as compared to the control 48-96 h after inoculation. Supero...Continue Reading

References

Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Jan 1, 1993·Free Radical Research Communications·N M AthertonJ T Törring
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·P L ConklinR L Last
Oct 1, 1996·The Plant Cell·K E Hammond-Kosack, J D Jones
Dec 22, 1999·The Plant Journal : for Cell and Molecular Biology·N DeightonB Williamson
Jul 13, 2000·Free Radical Biology & Medicine·L M Guan, J G Scandalios
May 17, 2003·Journal of Plant Physiology·Iwona MorkunasStefan Waplak
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Chris Lamb, Richard A. Dixon
Jan 1, 1995·Annual Review of Phytopathology·C J Baker, E W Orlandi

❮ Previous
Next ❯

Citations

Feb 13, 2016·Frontiers in Plant Science·Łukasz WojtylaMałgorzata Garnczarska
Dec 27, 2005·Journal of Plant Physiology·Iwona Morkunas, Joanna Gmerek
Mar 1, 2008·Plant Signaling & Behavior·Hayat El-Maarouf-Bouteau, Christophe Bailly
Aug 31, 2013·Plant Science : an International Journal of Experimental Plant Biology·Iwona MorkunasWaldemar Bednarski
Oct 25, 2016·International Journal of Molecular Sciences·Beata Borowiak-SobkowiakIwona Morkunas
Feb 7, 2017·International Journal of Molecular Sciences·Agnieszka WoźniakIwona Morkunas
Oct 22, 2019·Molecular Plant-microbe Interactions : MPMI·Davide GernaIlse Kranner
Jan 4, 2006·Mycopathologia·Margit SchollenbergerWinfried Drochner
Jul 29, 2019·Journal of Plant Physiology·Agnieszka WoźniakIwona Morkunas

❮ Previous
Next ❯