Abstract
Defence responses of embryo axes of Lupinus luteus L. cv. Polo were studied 48-96 h after inoculation with Fusarium oxysporum Schlecht f.sp. lupini. The infection restricted the growth of embryo axes, the lengths of infected embryo axes 72 and 96 h after inoculation were 11 and 12 mm less in the controls, respectively, while their masses c. 0.03 g less than in the controls. The concentration of H2O2 in embryo axes of inoculated germinating seeds was higher than in the control. This was probably a consequence of oxidative burst as well as H2O2 generation by the invading necrotrophic fungal pathogen. EPR-based analyses detected the presence of free radicals with g1 and g2 values of 2.0052 +/- 0.0004 and 2.0031 +/- 0.0005, respectively. Concentrations of the radicals 72 and 96 h after inoculation were 50% higher than in the control. The values of the spectroscopic splitting coefficients suggest that they are quinone radicals. However, inoculated embryo axes possess a number of adaptive mechanisms protecting them from oxidative damage. A twofold increase in catalase (CAT, EC 1.11.1.6) activity was evidenced in embryo axes infected with F. oxysporum Schlecht f. sp. lupini, as compared to the control 48-96 h after inoculation. Supero...Continue Reading
References
May 7, 1976·Analytical Biochemistry·M M Bradford
Jan 1, 1990·Free Radical Biology & Medicine·D R Janero
Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Nov 18, 1994·Cell·A LevineC Lamb
Jan 1, 1993·Free Radical Research Communications·N M AthertonJ T Törring
Sep 1, 1993·European Journal of Biochemistry·D BartlingE W Weiler
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·P L ConklinR L Last
Oct 1, 1996·The Plant Cell·K E Hammond-Kosack, J D Jones
Nov 7, 1996·Gene·S LoprasertS Mongkolsuk
Dec 22, 1999·The Plant Journal : for Cell and Molecular Biology·N DeightonB Williamson
Jul 13, 2000·Free Radical Biology & Medicine·L M Guan, J G Scandalios
Apr 6, 2001·Planta·I MuckenschnabelN Deighton
Apr 12, 2001·Plant Physiology·P SchopferG Frahry
Aug 29, 2001·Phytochemistry·A M MayerN L Gil-ad
Jan 25, 2002·Journal of Experimental Botany·I MuckenschnabelN Deighton
May 17, 2003·Journal of Plant Physiology·Iwona MorkunasStefan Waplak
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Chris Lamb, Richard A. Dixon
Jan 1, 1995·Annual Review of Phytopathology·C J Baker, E W Orlandi
Citations
Jan 26, 2012·Protoplasma·Iwona MorkunasWaldemar Bednarski
Feb 12, 2014·Toxins·Agnieszka WaśkiewiczPiotr Goliński
Sep 2, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Magda FormelaIwona Morkunas
Feb 13, 2016·Frontiers in Plant Science·Łukasz WojtylaMałgorzata Garnczarska
Oct 5, 2007·Journal of Plant Physiology·Iwona Morkunas, Waldemar Bednarski
Oct 22, 2009·Plant, Cell & Environment·Thomas RoachIlse Kranner
Dec 27, 2005·Journal of Plant Physiology·Iwona Morkunas, Joanna Gmerek
Mar 1, 2008·Plant Signaling & Behavior·Hayat El-Maarouf-Bouteau, Christophe Bailly
Aug 31, 2013·Plant Science : an International Journal of Experimental Plant Biology·Iwona MorkunasWaldemar Bednarski
Oct 25, 2016·International Journal of Molecular Sciences·Beata Borowiak-SobkowiakIwona Morkunas
Feb 7, 2017·International Journal of Molecular Sciences·Agnieszka WoźniakIwona Morkunas
Oct 22, 2019·Molecular Plant-microbe Interactions : MPMI·Davide GernaIlse Kranner
Jan 4, 2006·Mycopathologia·Margit SchollenbergerWinfried Drochner
Jul 29, 2019·Journal of Plant Physiology·Agnieszka WoźniakIwona Morkunas