Response of hPDLSCs on 3D printed PCL/PLGA composite scaffolds in vitro

Molecular Medicine Reports
Caixia PengLiping Wu

Abstract

Three‑dimensional printed (3DP) scaffolds have become an excellent resource in alveolar bone regeneration. However, selecting suitable printable materials remains a challenge. In the present study, 3DP scaffolds were fabricated using three different ratios of poly (ε‑caprolactone) (PCL) and poly‑lactic‑co‑glycolic acid (PLGA), which were 0.1PCL/0.9PLGA, 0.5PCL/0.5PLGA and 0.9PCL/0.1PLGA. The surface characteristics and degradative properties of the scaffolds, and the response of human periodontal ligament stem cells (hPDLSCs) on the scaffolds, were assessed to examine the preferable ratio of PCL and PLGA for alveolar bone regeneration. The results demonstrated that the increased proportion of PLGA markedly accelerated the degradation, smoothed the surface and increased the wettability of the hybrid scaffold. Furthermore, the flow cytometry and Cell Counting Kit‑8 assay revealed that the adhesion and proliferation of hPDLSCs were markedlyincreased on the 0.5PCL/0.5PLGA and 0.1PCL/0.9PLGA scaffolds. Additionally, the alkaline phosphatase activity detection and reverse‑transcription quantitative polymerase chain reaction demonstrated that the hPDLSCs on the 0.5PCL/0.5PLGA scaffold exhibited the best osteogenic capacity. Consequent...Continue Reading

References

Jun 1, 1978·Nature·J Folkman, A Moscona
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 19, 2003·Journal of Biomedical Materials Research. Part a·Anil ThapaKaren M Haberstroh
Apr 30, 2005·Biomaterials·Vassilis Karageorgiou, David Kaplan
Jun 1, 2005·Journal of Biomedical Materials Research. Part a·G ZhaoB D Boyan
Jun 21, 2005·Nature Biotechnology·Shulamit LevenbergRobert Langer
Aug 19, 2010·Methods in Molecular Biology·Krzysztof MrozikP Mark Bartold
Aug 2, 2011·Journal of Materials Science. Materials in Medicine·Rose Ann FrancoByong-Taek Lee
May 12, 2012·Polymers·Hirenkumar K Makadia, Steven J Siegel
May 24, 2012·Annals of Biomedical Engineering·Anuradha SubramanianSwaminathan Sethuraman
Jan 25, 2013·International Journal of Nanomedicine·Tingli LuTao Chen
Feb 26, 2015·Journal of Tissue Engineering and Regenerative Medicine·Chen ZongJinfu Wang
Apr 11, 2015·Stem Cells International·Wenjun Zhu, Min Liang
Apr 14, 2015·Journal of Biological Engineering·Helena N Chia, Benjamin M Wu
Apr 29, 2015·Biomaterials·Todd JensenChristine Finck
Jun 21, 2015·Cytotechnology·Ieva BružauskaitėEiva Bernotienė
Jul 1, 2015·Journal of Dental Research·G RasperiniW V Giannobile
Jul 1, 2015·Journal of Dental Research·F ObregonL E Bertassoni
Sep 22, 2015·Annals of Maxillofacial Surgery·Chad M TevenRussell R Reid
Apr 30, 2016·Materials Science & Engineering. C, Materials for Biological Applications·V C C TrajanoM E Cortés
Jun 10, 2016·ACS Applied Materials & Interfaces·Yu FuYan-Heng Zhou
Jun 14, 2016·Annals of Biomedical Engineering·Ethan L NybergWarren L Grayson
Oct 19, 2016·Journal of the Mechanical Behavior of Biomedical Materials·Shih-Feng Chou, Kim A Woodrow
Dec 13, 2016·ACS Biomaterials Science & Engineering·Ben P HungWarren L Grayson
Feb 20, 2017·Clinical Oral Investigations·Raquel OsorioManuel Toledano
Mar 4, 2017·Materials Science & Engineering. C, Materials for Biological Applications·S SprianoS Ferraris
Apr 7, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Junyi LiaoWei Huang
Apr 19, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Praneetha PulyalaKetul C Popat
Sep 29, 2017·Journal of Functional Biomaterials·Mythili PrakasamLiga Berzina-Cimdina

❮ Previous
Next ❯

Citations

Apr 24, 2020·Journal of Cellular Physiology·Zhengyuan ChenLiping Wu
Jul 16, 2021·Biomedical Materials·Amrita Bds MTech NatarajanPrabha D Ph D Nair

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
scanning electron microscopy
flow cytometry
fluorescence-activated cell sorting
protein assay

Software Mentioned

SPSS
BD Cell Quest Pro
SCA20

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.