PMID: 8600371Mar 1, 1996Paper

Response of human keratinocytes to extremely low concentrations of N-methyl-N'-nitro-N-nitrosoguanidine

Mutation Research
H E Kleczkowska, F R Althaus

Abstract

Since alkylating agents are widely present in the environment and constitute a continuous challenge to genome integrity, cells and organisms have developed defense mechanisms to remove such lesions. We monitored the response of human keratinocytes to a very low concentration of a methylating agent, namely 2.5 nM N-methyl-N'-nitro- N-nitrosoguanidine (MNNG). The effect of a 60-min exposure of quiescent cells to 2.5 nM MNNG was studied in terms of DNA integrity, poly(ADP-ribose) metabolism, clonogenic survival and DNA synthesis. We observed two waves of DNA strand break formation and resealing. Interestingly, the amount of DNA strand breaks in exposed cells was lower than in unexposed control cells. This phenomenon was also observed when cells were exposed to MNNG in the presence of a protein synthesis inhibitor, or when they were maintained on ice during the treatment. A dose of 2.5 nM MNNG stimulated poly(ADP-ribose) turnover, reduced the intracellular NAD+ content, stimulated DNA synthesis and caused a remarkable increase in clonogenic survival. Thus, the cellular responses to extremely low concentrations of MNNG differ sharply from those observed at higher doses of this carcinogen. We conclude that the very low dose response ...Continue Reading

References

Jul 1, 1992·Chemical Research in Toxicology·D E Shuker, P B Farmer
Jun 25, 1992·Nucleic Acids Research·P Karran, M Bignami
Sep 1, 1991·Experimental Cell Research·M E Boerrigter
Aug 11, 1989·Science·L A Sagan
Mar 1, 1987·Mutation Research·J Harless, R R Hewitt
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J W Park, B N Ames
Oct 1, 1988·Analytical Biochemistry·N Aboul-ElaM K Jacobson
Nov 1, 1986·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·E Dikomey, J Franzke
Sep 1, 1987·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·F H SchneeweissL E Feinendegen
Oct 1, 1987·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·I R Radford
Aug 1, 1986·Analytical Biochemistry·R Alvarez-GonzalezF R Althaus
Jan 1, 1987·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S E ShephardW K Lutz
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Jan 1, 1982·Carcinogenesis·L R Barrows, P N Magee
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W Xiao, L Samson
Jan 1, 1994·Methods in Enzymology·C Baumstark-Khan
Sep 9, 1994·Science·P H Abelson
Nov 1, 1993·International Journal of Radiation Biology·H Huang, H G Claycamp
Jun 1, 1994·Cancer Metastasis Reviews·P L Olive, R E Durand
Apr 1, 1994·Trends in Biochemical Sciences·G de Murcia, J Ménissier de Murcia
Jan 1, 1993·Progress in Nucleic Acid Research and Molecular Biology·S Mitra, B Kaina

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Citations

Nov 4, 1996·Mutation Research·H E Kleczkowska, F R Althaus
Feb 22, 2007·Mutagenesis·Lucyna KapkaDanuta Mielzyńska
Apr 15, 2000·Risk Analysis : an Official Publication of the Society for Risk Analysis·E J CalabreseC D Holland
Aug 6, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jana PeremartíAlba Hernández
Jun 15, 1999·Biulleten' eksperimental'noĭ biologii i meditsiny·G D ZasukhinaG N L'vova

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