Response of human macrophage-like cells to stimulation by Fusobacterium nucleatum ssp. nucleatum lipopolysaccharide

Oral Microbiology and Immunology
D Grenier, L Grignon

Abstract

Monocytes/macrophages are key members of the innate immune system and are present in higher numbers in active periodontal lesions than in inactive sites. The aim of this study was to characterize the response of human monocyte U937 cells, differentiated into adherent macrophages by treatment with phorbol-12-myristate 13-acetate, to stimulation by Fusobacterium nucleatum ssp. nucleatum lipopolysaccharide. Attachment of (3)H-lipopolysaccharide to macrophage-like cells was partially inhibited by anti-CD14 and anti-TLR4 polyclonal antibodies. Fusobacterial lipopolysaccharide did not cause cell apoptosis or block apoptosis induced by camptothecin. Lipopolysaccharide up-regulated the secretion of the pro-inflammatory cytokines interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha as well as the chemokine interleukin-8 by macrophage-like cells. In addition, it increased phospholipase C and D activities, which likely contributed to the high levels of prostaglandin E(2) detected in the cell culture supernatant. Lastly, the amount of matrix metalloproteinase-9 produced by macrophage-like cells was significantly increased by the lipopolysaccharide treatment. Interestingly, fusobacterial cells acquired matrix metalloproteinase-...Continue Reading

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Citations

Jul 12, 2013·PloS One·Tao YanJieli Chen
Mar 9, 2010·Experimental Lung Research·Karen C YoungCleide Suguihara
Nov 11, 2009·Atherosclerosis·Wijnand K den DekkerHencricus J Duckers
Dec 2, 2008·Tissue & Cell·R YounesN Naaman
Mar 21, 2007·Journal of Cellular and Molecular Medicine·Hongli Li, Baogui Sun
Dec 18, 2008·The Open Dentistry Journal·Guang Jie BaoJukka H Meurman

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