Response of human oligodendrocytes to interleukin-2

Brain, Behavior, and Immunity
G C Otero, J E Merrill

Abstract

Interleukin 2 (IL-2) directly affects the function of both neurons and glia in the nervous system. It can induce proliferation and differentiation or cause cell death in oligodendrocytes. We have previously cloned the cDNAs for the alpha (alpha), beta (beta), and gamma (gamma) chains of the IL-2 receptor (IL-2R) complex from a human oligodendroglioma cell line TC620. In an effort to characterize the IL-2 receptor (IL-2R) on oligodendrocytes, experiments were performed using recombinant human IL-2 on normal human oligodendrocytes from adult brain tissue and the IL-2-responsive subclone TC620.6A2 of the oligodendroglioma line. The TC620 subclone has the phenotype of an immature oligodendrocyte. At 5 nM IL-2, there was a 2.5-fold increase in proliferation of both normal and malignant human oligodendrocytes. This response was receptor-mediated in that binding of 125I-IL-2 to TC620.6A2 cells detected a single receptor class for IL-2 with an affinity of 3.6 nM. Immunohistochemical staining of TC620.6A2 cells with a panel of monoclonal antibodies to different epitopes of the human IL-2R alpha chain demonstrated the presence of IL-2R alpha on the surface of these cells, in staining patterns which did not always coincide with those foun...Continue Reading

Citations

Jan 13, 2000·Neuropathology and Applied Neurobiology·J E Merrill, N J Scolding
Feb 25, 2009·Annals of the New York Academy of Sciences·Elizabeth Giestal de AraujoAline Araujo Dos Santos
Jun 30, 2007·The American Journal of Pathology·Fiona H AmanteChristian R Engwerda
Sep 10, 2014·Cytokine & Growth Factor Reviews·Geok Choo Sim, Laszlo Radvanyi
Oct 12, 2001·Glia·T BennN Scolding
Sep 19, 2008·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jean E Merrill
Mar 29, 2001·Physiological Reviews·N Baumann, D Pham-Dinh
Jan 19, 2021·Experimental Eye Research·S A Espírito-SantoP Campello-Costa

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