Response of human peritoneal mesothelial cells to inflammatory injury is regulated by interleukin-1beta and tumor necrosis factor-alpha

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
S StadlmannAlbert Amberger

Abstract

Peritoneal injury is often associated with alterations of the mesothelium, resulting in peritoneal healing and adhesion formation. We analyzed the effects of pro-inflammatory cytokines on cell morphology and proliferation of human peritoneal mesothelial cells (HPMC). After 48 hours, HPMC formed a confluent layer with cell volumes of 2,662+/-111 fL. Treatment of HPMC with interleukin-1beta and tumor necrosis factor-alpha (TNF-alpha) induced mesothelial disintegration and alterations in mesothelial cell morphology, which were associated with an interleukin-1beta-triggered increase in cell volume (3,028+/-118 fL; p<0.05) and exfoliation of cells into the supernatants of cell cultures (p<0.05). Whereas TNF-alpha arrested HPMC in the G0/G1 phase (p<0.05), interleukin-1beta caused an increase of cells into the S phase of the cell cycle. In addition, interleukin-1beta and interferon-gamma exerted a proliferative effect on HPMC. These changes were independent from mesothelial Na+/H+ antiporter-1 expression. Our data indicate that the response of HPMC to inflammatory injury is regulated by interleukin-1beta and TNF-alpha reflecting their putative role in peritoneal wound healing and adhesion formation.

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Citations

Sep 18, 2013·Clinical & Experimental Metastasis·Ying DongJudith A Clements
Jul 15, 2009·Journal of Molecular Neuroscience : MN·Xiaorong XinHanspeter E Killer
Jan 9, 2015·Scientific Reports·Tatsuya SuzukiHiroyuki Furukawa
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Jun 17, 2015·BioMed Research International·Mari MatobaAkeo Hagiwara
Nov 30, 2006·Annals of the New York Academy of Sciences·Robert G GourdieAndrew W Hunter

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