Response of human REV1 to different DNA damage: preferential dCMP insertion opposite the lesion

Nucleic Acids Research
Yanbin ZhangZhigang Wang

Abstract

REV1 functions in the DNA polymerase zeta mutagenesis pathway. To help understand the role of REV1 in lesion bypass, we have examined activities of purified human REV1 opposite various template bases and several different DNA lesions. Lacking a 3'-->5' proofreading exonuclease activity, purified human REV1 exhibited a DNA polymerase activity on a repeating template G sequence, but catalyzed nucleotide insertion with 6-fold lower efficiency opposite a template A and 19-27-fold lower efficiency opposite a template T or C. Furthermore, dCMP insertion was greatly preferred regardless of the specific template base. Human REV1 inserted a dCMP efficiently opposite a template 8-oxoguanine, (+)-trans-anti-benzo[a]pyrene-N2-dG, (-)-trans-anti-benzo[a]pyrene-N2-dG and 1,N6-ethenoadenine adducts, very inefficiently opposite an acetylaminofluorene-adducted guanine, but was unresponsive to a template TT dimer or TT (6-4) photoproduct. Surprisingly, the REV1 specificity of nucleotide insertion was very similar in response to different DNA lesions with greatly preferred C insertion and least frequent A insertion. By combining the dCMP insertion activity of human REV1 with the extension synthesis activity of human polymerase kappa, bypass of th...Continue Reading

References

Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M H KokenJ H Hoeijmakers
Jan 1, 1995·Journal of Occupational and Environmental Medicine·K Peltonen, A Dipple
Aug 11, 1995·Journal of Molecular Biology·P E Gibbs, C W Lawrence
Jan 1, 1994·Drug Metabolism Reviews·H BartschY Guichard
Jun 14, 1996·Science·J R NelsonD C Hinkle
Aug 22, 1996·Nature·J R NelsonD C Hinkle
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·P E GibbsC W Lawrence
Mar 5, 1999·Mutation Research·J NairH Bartsch
Jul 10, 1999·Science·R E JohnsonL Prakash
Oct 28, 1999·Nucleic Acids Research·W LinZ Wang
Mar 14, 2000·The Journal of Biological Chemistry·F YuanZ Wang
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·P E GibbsV M Maher
Jul 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·S TateishiM Yamaizumi
Jul 25, 2000·Nucleic Acids Research·H XinZ Wang
Nov 30, 2000·Nucleic Acids Research·Y ZhangZ Wang
Feb 13, 2001·Nucleic Acids Research·Y ZhangZ Wang
Apr 12, 2001·The Journal of Biological Chemistry·T IshikawaT Todo
May 29, 2001·The Journal of Biological Chemistry·R L LevineM Moriya
Aug 4, 2001·The Journal of Biological Chemistry·Y MurakumoM Takahashi
Aug 23, 2001·Molecular Cell·H OhmoriR Woodgate

❮ Previous
Next ❯

Citations

Jan 17, 2004·Biochimie·P Kannouche, A Stary
Dec 3, 2002·Mutation Research·G P Holmquist, Veronica M Maher
Dec 3, 2002·Mutation Research·Alexandra VaismanRoger Woodgate
Sep 19, 2003·DNA Repair·Jacob G Jansen, Niels de Wind
Feb 14, 2012·Chemical Research in Toxicology·Shanen M SherrerZucai Suo
Feb 16, 2012·Chemical Research in Toxicology·Alex R KlugIrina G Minko
Mar 15, 2008·Nature Reviews. Immunology·Jean-Claude Weill, Claude-Agnès Reynaud
Feb 24, 2012·Nature Reviews. Molecular Cell Biology·Julian E SaleRoger Woodgate
Jul 2, 2008·The Journal of Biological Chemistry·Jeong-Yun Choi, F Peter Guengerich
Feb 16, 2006·The Journal of Experimental Medicine·Jacob G JansenNiels de Wind
Aug 22, 2003·Nucleic Acids Research·Denise R ClarkW Glenn McGregor
Jul 31, 2004·Nucleic Acids Research·Bo ZhaoZhigang Wang
Jan 18, 2005·Nucleic Acids Research·Jacob G JansenNiels de Wind
Nov 15, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Julian E SaleDávid Szüts
Oct 30, 2010·Plant Physiology·Mayu NakagawaAyako N Sakamoto
Apr 1, 2009·Molecular and Cellular Biology·Jacob G JansenNiels de Wind
Sep 20, 2006·Molecular and Cellular Biology·Caixia GuoErrol C Friedberg
Jun 22, 2011·Molecular and Cellular Biology·Franklin Mayca PozoTakayuki Yamashita
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Lauren S WatersGraham C Walker
Jan 30, 2009·Molecular Cancer Research : MCR·Chad A DumstorfW Glenn McGregor
Oct 29, 2010·Genetics·Mary Ellen Wiltrout, Graham C Walker
Oct 28, 2006·Radiation Research·Joann B SweasyKristin A Eckert
Nov 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tomoo OgiHaruo Ohmori

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