Response of intestinal flora of laboratory-reared leopard frogs (Rana pipiens) to cold and fasting.

Applied and Environmental Microbiology
J GosslingG W Nace

Abstract

The bacterial flora of the large intestine was examined in 35 laboratory-reared leopard frogs (Rana pipiens) subjected to one of the following four treatments: (i) normal feeding at 21 degrees C (10 frogs); (ii) fasting for 2 weeks at 21 degrees C (8 frogs); (iii) chilling for 1 week at 4 degrees C (9 frogs); and (iv) simulated hibernation for 3 weeks at 4 degrees C (8 frogs). Bacteria from the intestinal contents and mucosa were counted microscopically and by colony counting after strictly anaerobic culturing. The predominant bacteria were isolated and partially characterized. Fasting for 2 weeks produced no significant changes in total counts or in the types of bacteria cultured. Chilling, whether rapid or in the course of simulated hibernation, was associated with a decrease in the numbers and variety of bacteria. Thus it appears that the lowering of temperature rather than the absence of food is the important factor in the reduction of bacterial flora seen in hibernating frogs. However, the bacteria showed some adaptation to the low temperature, as the longer the host had been at 4 degrees C, the higher the proportion of bacteria which could grow when cultured at that temperature.

References

Mar 1, 1975·The Journal of Pediatrics·M E Ament
Jan 1, 1977·Annual Review of Microbiology·D C Savage
Nov 1, 1970·The American Journal of Clinical Nutrition·H Haenel
Jul 1, 1982·Applied and Environmental Microbiology·J GosslingG W Nace

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Citations

Mar 5, 2016·Environmental Microbiology·Kevin D Kohl, Jeremiah Yahn
May 4, 2018·Proceedings. Biological Sciences·James M WieblerJon P Costanzo
Nov 7, 2019·Science China. Life Sciences·Jiaying LiXiangzhen Li
Mar 12, 2016·BMC Microbiology·Chun-Wen ChangPei-Chun Liao
Nov 11, 2015·Microbiome·Irene Hanning, Sandra Diaz-Sanchez
Aug 25, 2009·Applied and Environmental Microbiology·Kei SonoyamaTatsuya Morita
Oct 9, 2014·Zoo Biology·Gina M FerrieEduardo V Valdes
Dec 1, 1983·Applied and Environmental Microbiology·D W HirdM Gasiorowski
Jan 12, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Marshall D McCue

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