Response of Methanogenic Microbial Communities to Desiccation Stress in Flooded and Rain-Fed Paddy Soil from Thailand

Frontiers in Microbiology
Andreas ReimR Conrad

Abstract

Rice paddies in central Thailand are flooded either by irrigation (irrigated rice) or by rain (rain-fed rice). The paddy soils and their microbial communities thus experience permanent or arbitrary submergence, respectively. Since methane production depends on anaerobic conditions, we hypothesized that structure and function of the methanogenic microbial communities are different in irrigated and rain-fed paddies and react differently upon desiccation stress. We determined rates and relative proportions of hydrogenotrophic and aceticlastic methanogenesis before and after short-term drying of soil samples from replicate fields. The methanogenic pathway was determined by analyzing concentrations and δ13C of organic carbon and of CH4 and CO2 produced in the presence and absence of methyl fluoride, an inhibitor of aceticlastic methanogenesis. We also determined the abundance (qPCR) of genes and transcripts of bacterial 16S rRNA, archaeal 16S rRNA and methanogenic mcrA (coding for a subunit of the methyl coenzyme M reductase) and the composition of these microbial communities by T-RFLP fingerprinting and/or Illumina deep sequencing. The abundances of genes and transcripts were similar in irrigated and rain-fed paddy soil. They also ...Continue Reading

References

Dec 3, 1998·Applied and Environmental Microbiology· Gro kopf RW Liesack
Jul 6, 2000·Applied and Environmental Microbiology·T Lueders, M Friedrich
Apr 11, 2001·Journal of Microbiological Methods·H BürgmannJ Zeyer
Feb 3, 2005·Environmental Microbiology·Matthias NollWerner Liesack
Apr 20, 2006·Current Opinion in Biotechnology·Ralf ConradWerner Liesack
Mar 18, 2008·Applied and Environmental Microbiology·Jingjing PengYahai Lu
Jan 28, 2009·Environmental Microbiology·Pravin Malla ShresthaWerner Liesack
May 26, 2009·Applied and Environmental Microbiology·Junpeng RuiYahai Lu
Dec 21, 2010·Applied and Environmental Microbiology·Scott T BatesNoah Fierer
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
Jun 1, 2011·Environmental Microbiology Reports·Yanli YuanYahai Lu
Nov 19, 2013·Environmental Microbiology·Nicholas D YoungblutRachel J Whitaker
Nov 30, 2014·Applied Microbiology and Biotechnology·Jingxu ZhangYong Liu
Jan 27, 2015·Applied and Environmental Microbiology·Marcela HernándezRalf Conrad
Feb 26, 2015·Environmental Microbiology·Carl-Eric Wegner, Werner Liesack
Mar 3, 2015·Environmental Microbiology Reports·Zhe Lyu, Yahai Lu
Mar 11, 2015·Environmental Microbiology Reports·Tina WunderlinPilar Junier
Apr 22, 2015·PloS One·Yongkyu Kim, Werner Liesack
Jan 23, 2016·Frontiers in Microbiology·Björn BreidenbachMarc G Dumont

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Citations

Apr 19, 2018·International Journal of Microbiology·Michele PittolVictor Hugo Valiati
Mar 28, 2019·Frontiers in Microbiology·Pengfei LiuRalf Conrad
Dec 31, 2019·Environmental Science and Pollution Research International·Yan-Qin WangZhi-Feng Zhou

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Datasets Mentioned

BETA
PRJNA362529
PRJNA362531

Methods Mentioned

BETA
PCR
PCRs
PCA
Illumina sequencing
soil treatment

Software Mentioned

mothur
UPARSE
cutadapt
UCHIME
Excel
vegan
R
gplots

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